Objective: To research the blood perfusion characteristic of non-alcoholic fatty liver disease (NAFLD) using multisection dynamic CT. Methods: Dynamic 64-slice computed tomography scans of the liver were obtained in 72 individuals including 20 control subjects,52 patients with NAFLD from October 2009 to June 2010. The multisection dynamic CT perfusion series were performed by a multisection CT scanner (Philip Brilliance 64),120 kV,250 mAs. The data were processed on a Philip Brilliance 64 workstation using perfusion CT software package,and the mean HAP,PVP,TLP,and HPI were measured and statistically analyzed in 72 subjects. Results: Liver perfusion (TPL and PVP) in patients with NAFLD decreased (P < 0.05) and HPI increased significantly (P < 0.05). Conclusion: Hepatic perfusion parameters measured with CT were significantly altered in NAFLD. Dynamic 64-slice CT is potential in predicting prognosis of patients with NAFLD.