蟾蜍灵对阿霉素肾病大鼠蛋白尿的影响及机制研究
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江苏省“六大高峰人才”项目资助(06-B-020)


Effect of bufalin against proteinuria of adriamycin-induced nephritic rats and its underlying mechanisms
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    目的:研究蟾蜍灵对阿霉素肾病大鼠蛋白尿的影响及相关机制-方法:40只SD大鼠随机分成3组:正常组-模型组和蟾蜍灵组,除正常组外大鼠尾静脉一次性注射阿霉素7.5 mg/kg,1周后,蟾蜍灵组每天腹腔注射蟾蜍灵0.1 mg/kg,持续3周,每周检测24 h尿蛋白定量,治疗3周后处死所有大鼠,全自动生化检测仪检测血清白蛋白-甘油三酯等相关指标,比色法检测血清氧化应激水平,光镜和电镜观察肾小球病理改变,Western blot检测肾皮质中整合素连接激酶(ILK)蛋白的表达-结果:蟾蜍灵能够降低阿霉素肾病大鼠24 h尿蛋白和血脂水平,减轻足细胞病变,抑制过氧化反应,降低肾皮质中ILK的高表达-结论:蟾蜍灵通过保护足细胞,减轻阿霉素肾病大鼠蛋白尿水平,而其抵抗足细胞损伤的机制可能与其降低氧化应激水平-下调ILK蛋白的表达量相关-

    Abstract:

    Objective: To investigate the effect of bufalin against proteinuria in adriamycin (ADR)-induced nephritic rats and its possible mechanisms. Methods: Forty SD rats were randomly divided into three groups: control group,model group and bufalin group. At the beginning,7.5 mg/kg ADR was singly administered through the tail vein except for the control rats. After 7 days,the rats in bufalin group were injected intraperitoneally with 0.1 mg/kg bufalin everyday unitl day 28. Total 24-h urine samples of all groups were collected weekly. At the end,biological parameters and oxidative stress in blood serum were measured,the histopathology of the renal cortex were detected by light microscopy and transmission electron microscopy,the expression of integrin-linked kinase(ILK) was qualified by Western blot. Results: Bufalin could reduce the 24-h urinary protein and blood lipid,alleviate the podocyte damage,inhibit the oxidative stress and down-regulate ILK in the protein level in ADR rats. Conclusion: Bufalin may against proteinuria by protecting podocyte,and its possible mechanisms against podocyte damage are related to inhibit the oxidative stress and down-regulate ILK in the protein level.

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郑 君,龚 晶,张爱青,李善文,曾志凤,甘卫华.蟾蜍灵对阿霉素肾病大鼠蛋白尿的影响及机制研究[J].南京医科大学学报(自然科学版),2011,(11):1605-1609

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  • 收稿日期:2011-06-07
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