IL-13对人结肠癌LS174T细胞增殖和侵袭能力的影响
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南京市医学科技发展项目(YKK10096)


The effects of IL-13 on proliferation and invasion potential of human colon cancer LS174T cells
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    目的:探讨细胞因子IL-13对人结肠癌LS174T细胞增殖和侵袭能力的影响-方法:在正常培养的人结肠癌LS174T细胞培养液中加入终浓度为20 ng/ml的重组人IL-13,分别应用MTT法-Transwell小室实验检测肿瘤细胞增殖-侵袭能力的变化-结果:MTT实验显示,IL-13处理组细胞72-96 h的OD值明显高于对照组(P < 0.05)-Transwell小室实验中,IL-13处理组细胞穿过Matrigel胶的细胞数量明显多于对照组(P < 0.05)-结论:一定浓度的重组人IL-13可以诱导人结肠癌LS174T细胞的增殖和侵袭能力增加-

    Abstract:

    Objective: To investigate the effects of cytokine IL-13 on proliferation and invasion of human colon cancer LS174T cells. Methods: LS174T cells were treated with 20 ng/ml recombinant human IL-13 as final concentration. The proliferative and invasive abilities of the cells were determined by MTT assay and Transwell invasion assay,respectively. Results: The MTT result showed that the proliferative capacity of IL-13 treatment group was significantly higher than that of control group(P < 0.05). IL-13 treatment group produced significantly more invasive cells than that of control group. Conclusion: Recombinant human IL-13 at a certain concentration could induce the proliferation and invasion of LS174T cells.

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李 俐,田智丹,黄文斌,王劲松,赵建华. IL-13对人结肠癌LS174T细胞增殖和侵袭能力的影响[J].南京医科大学学报(自然科学版),2011,(12):1823-1825

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  • 收稿日期:2011-07-17
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