性激素对女性脂肪组织11βHSD mRNA表达的部位特异性调控
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国家科学自然基金(30672269)


The depot-specific regulation of sex hormones on 11βHSD1 mRNA expression in adipose tissue
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    目的:观察性激素对育龄期和绝经期妇女不同部位脂肪组织11β羟基类固醇脱氢酶Ⅰ型(11β-hydroxysteroid dehydrogenase type 1,11βHSD1)mRNA表达水平的调控作用-方法:育龄期非妊娠妇女6例,年龄(29.83 ± 3.43)岁及绝经期妇女16例,年龄(65.19 ± 6.81)岁,取其皮下和网膜脂肪组织进行培养,应用雌激素和雄激素刺激脂肪组织24h,实时定量PCR方法测定脂肪组织11βHSD1 mRNA水平-结果:雌激素可使绝经期妇女皮下脂肪组织11βHSD1 mRNA表达明显增加(P < 0.05),使网膜脂肪组织11βHSD1 mRNA表达水平下降30%-雄激素刺激可抑制育龄期妇女皮下脂肪组织11βHSD1 mRNA表达近40%(P = 0.001),而使绝经期妇女网膜脂肪组织11βHSD1 mRNA表达增加1.9倍(P = 0.005)-结论:性激素对脂肪组织11βHSD1 mRNA表达的调控呈现年龄和部位特异性改变,可能是不同时期女性体脂分布的重要分子生物学机制-

    Abstract:

    Objective: To observe the depot-specific regulation of sex hormones on 11β-hydroxysteroid dehydrogenase type 1(11βHSD1) mRNA expression in adipose tissue in child-bearing and menopausal women. Methods: Subcutaneous(Sc) and omental(Om) adipose tissues were obtained from 6 child-bearing(aged 29.83 ± 3.43 years) and 16 menopausal women(aged 65.19 ± 6.81 years). Pieces of adipose tissue were incubated with estrogen or testosterone for 24h. The expression of 11βHSD1 mRNA in adipose tissues was measured by real time PCR. Results: Estrogen increased 11βHSD1 mRNA expression in Sc adipose tissue (P < 0.05) but decreased 11βHSD1 mRNA expression in Om adipose tissue by 30% among menopausal women. Androgen decreased 11βHSD1 mRNA expression in Sc adipose tissue of child-bearing period women by 40%(P = 0.001),and up-regulated 11βHSD1 mRNA expression in Om adipose tissue of menopausal women by 1.9 fold(P = 0.005). Conclusion: The sex hormones stimulate 11βHSD1 mRNA expression in adipose tissue in age- and depot-specific pattern,which may be important molecular mechanism of distribution of body fat in child-bearing period and menopausal women.

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王 晶,张 丽,沈宇飞,韩树萍,李晓南.性激素对女性脂肪组织11βHSD mRNA表达的部位特异性调控[J].南京医科大学学报(自然科学版),2012,(1):53-57

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  • 收稿日期:2011-08-31
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