基于管家基因和水平转移基因的菌株亲缘性分析:60株耐药大肠埃希菌研究
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江苏省实验诊断学重点实验室重大项目(XK200731)


The relatedness in 60 drug-resistant Escherichia coli strains based on the analysis of the housekeeping genes and genes acquired by horizontal transfer
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    摘要:

    目的:了解1组(60株)耐药大肠埃希菌的菌株亲缘性-方法:对该组耐药大肠埃希菌4种与耐药相关的管家基因和45种水平转移获得与β-内酰胺类-氨基糖苷类-喹诺酮类耐药相关基因以及7种整合子-转座子-插入序列-接合性质粒遗传标记进行检测-并对检测结果进行样本聚类分析-结果:60株耐药大肠埃希菌多态性明显,多数菌株已发生演化-只有6-53号株;10-14号株,7-8-26-40号株3组携带相同基因(同一克隆)-结论:耐药基因的样本聚类分析可识别出具有相同耐药特征的菌株,具有一定的流行病学意义,为追溯耐药菌传播途径提供方便-

    Abstract:

    Objective: To investigate the relatedness in 60 drug-resistant Escherichia coli(E.coli) strains. Methods: Drug-resistance related genes were analyzed by PCR,including 4 kinds of housekeeping genes and 45 kinds of horizontal transfered genes related to 3 kinds of antimicrobial agents:beta-lactams,aminoglycosides,quinolones,as well as 7 kinds of genetic markers of integrons,transposons,insertion sequences and conjugal plasmids. They were used for the sample clustering analysis. Results: Polymorphism were distinct in 60 E.coli strains. Most strains showed evolution. Only 3 strain groups which consisted of strain 6 and 53,strain 10 and 14,strain 7,8,26 and 40,were the same clones carried the same genes. Conclusion: Sample clustering analysis of drug-resistance related genes could distinguish the strains with the same antimicrobial resistance characteristics,which was significant in epidemiologic research and provide convenience for tracing transmissible routes of drug-resistant strains.

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赵旺胜,王 芳,翁幸鐾,糜祖煌,潘世扬.基于管家基因和水平转移基因的菌株亲缘性分析:60株耐药大肠埃希菌研究[J].南京医科大学学报(自然科学版),2012,(1):109-114

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  • 收稿日期:2011-07-15
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