肉鸡胴体中沙门氏菌的分离鉴定及多重耐药谱研究
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卫生部公益性行业科研专项基金(200902009)


Separation and identification of multiple-antimicrobial-resistant of Salmonella on chicken carcass
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    目的:为了解肉鸡胴体中沙门氏菌的污染状况-多重耐药状况及耐药程度,对样品中的沙门氏菌进行分离鉴定和药敏试验研究-方法:根据GB/T 4789—2008对沙门氏菌进行分离鉴定,采用微量肉汤稀释法测定分离株的耐药性-结果:肉鸡胴体沙门氏菌的检出率高达34.8%;样品中分离的沙门氏菌血清型主要为奥尔巴尼型(29.6%)和印第安纳型(50.7%);药敏试验显示,大多数沙门氏菌至少对3种以上的抗生素产生耐药,耐3~7种药物的有24株(33.8%),耐8~12种药物的有19株(26.8%),耐13~16种药物的有26株(36.6%);分离株对抗生素的耐药情况是:萘啶酸(100%)-磺胺嘧啶(98.6%)-氨苄青霉素(97.2%)-复方磺胺甲恶唑(85.9%)-四环素(85.9%)和阿莫西林/克拉维酸(84.5%)-结论:肉鸡胴体中沙门氏菌的污染现象严重,沙门氏菌耐药或多重耐药现象十分普遍,未来应加强对鸡胴体中沙门氏菌感染及耐药的监测-

    Abstract:

    Objective: To determine prevalence and multiple-antimicrobial-resistant spectrum of Salmonella contamination on chicken carcasses. Methods: According to GB 4789-2008,Salmonellas were separated and identified,and drug resistance of the strains was analyzed using broth dilution method. Results: Salmonella was detected in 34.8%(71/204) of samples. Two Salmonella serovars were identified;predominant ones included Albany(29.6%) and Indiana(50.7%). Most of the Salmonella isolates tested,exhibited resistance to more than three antimicrobial agents used. Twenty-four strains(33.8%) were resistant to three to seven kinds,nineteen strains(26.8%) were resistant to eight to twelve,and twenty-six strains(36.6%) resistant to thirteen and sixteen antibiotics. Resistance to the following antibiotics was common among isolates:nalidixic acid (100%),sulphadiazine(98.6%),ampicillin (97.2%),sulfamethoxazole(85.9%),minocycline (85.9%) and amoxicillin/clavulanic acid (84.5%). Conclusion: High prevalence of Salmonella and the relatively high resistance among the bacteria suggested monitoring of the Salmonella infection and drug resistance on chicken carcass should be strengthened in the future.

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吴云凤,袁宝君,乔 昕,符晓梅,沈 贇,王燕梅,马 恺,倪云龙.肉鸡胴体中沙门氏菌的分离鉴定及多重耐药谱研究[J].南京医科大学学报(自然科学版),2012,(1):125-128

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  • 收稿日期:2011-07-11
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