Objective:To investigate the role of aquaporin-4(AQP4) on migration and proliferation of human colon cancer cell. Methods: AQP4 expression was determined by Western blot after vascular endothelial growth factor(VEGF) or AQP4-siRNA treatment in human colon cancer cell line LoVo,and the cell migration and proliferation were tested by cell scratch assay and CCK-8 proliferation assay. Results: VEGF up-regulated AQP4 expression of LoVo cell in a time- or concentration-dependent manner,and increased cell migration. When AQP4 was knocked down by AQP4-siRNA,cell migration decreased significantly. Compared with the VEGF-treated cell,the migration of combined AQP4-siRNA and VEGF treated cell decreased. AQP4-siRNA or VEGF had no significant effect on cell proliferation. Conclusion: AQP4 may promote the migration of human colon cancer cell. This provide evidence for a novel role of AQP4 in tumor growth and spread of human colon cancer.