S100A16转基因小鼠的构建与鉴定
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金(81070684),江苏省科技支撑计划(BE2011802),江苏省“六大人才高峰”项目,南京医科大学第一附属医院创新团队工程资助


Establishment and identification of S100A16 transgenic mice
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:构建系统表达S100A16基因型小鼠,为研究S100A16基因的生物学功能提供模型动物-方法:将S100A16 cDNA插入CMV启动子下游,构建转基因表达载体,通过显微注射方法建立转基因小鼠-PCR鉴定转基因小鼠的基因型,采用定量PCR(QPCR)方法筛选高表达品系-结果:成功构建S100A16转基因载体,建立了S100A16转基因小鼠,通过PCR及QPCR方法筛选出两个高表达品系(line A,line B)-结论:建立了系统表达S100A16的转基因小鼠,转入的S100A16基因在脂肪-肝脏-肌肉-肺等组织高表达,为研究S100A16的生物学功能特别是在肥胖中的作用及机制提供了动物模型-

    Abstract:

    Objective:To establish a transgenic mouse model systemic expression S100A16,which can be used for the study of its biologic function. Methods: The plasmid was generated by inserting the murine S100A16 cDNA into a vector with CMV promoter. The transgenic mice were produced by the method of microinjection. The genotype of transgenic lines was identified by PCR and the expression level of the gene was determined by QPCR. Results: S100A16 cDNA transgenic vector was successfully constructed and S100A16 transgenic mice were created. Two high expression lines were determined by QPCR. Conclusion: The transgenic mouse systemic expressing S100A16 was successfully established,and the S100A16 gene was highly expressed in many tissues,such as adipose tissue,liver,muscles,and lung. The S100A16 mouse could be a useful model for the researches of its function,especially in obesity and insulin resistance.

    参考文献
    相似文献
    引证文献
引用本文

杜新丽,薛 一,张媛媛,张日华,刘 云. S100A16转基因小鼠的构建与鉴定[J].南京医科大学学报(自然科学版),2012,(3):325-328

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2011-10-01
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期:
通知关闭
郑重声明