Objective:To establish a transgenic mouse model systemic expression S100A16,which can be used for the study of its biologic function. Methods: The plasmid was generated by inserting the murine S100A16 cDNA into a vector with CMV promoter. The transgenic mice were produced by the method of microinjection. The genotype of transgenic lines was identified by PCR and the expression level of the gene was determined by QPCR. Results: S100A16 cDNA transgenic vector was successfully constructed and S100A16 transgenic mice were created. Two high expression lines were determined by QPCR. Conclusion: The transgenic mouse systemic expressing S100A16 was successfully established,and the S100A16 gene was highly expressed in many tissues,such as adipose tissue,liver,muscles,and lung. The S100A16 mouse could be a useful model for the researches of its function,especially in obesity and insulin resistance.