汉防己甲素联合紫杉醇对胃癌细胞增殖和凋亡的影响
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国家自然科学基金(81001077);江苏省卫生厅"135工程"医学重点人才基金(RC2007046)


Effects of tetrandrine in combination with paclitaxel on the proliferation and apoptosis of human gastric cancer cell line
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    摘要:

    目的:研究汉防己甲素(tetrandrine,TET)联合化疗药物紫杉醇(paclitaxel,PTX)对人胃癌细胞增殖和凋亡的影响-方法:MKN-45细胞常规培养于RPMI-1640培养液中,细胞长至亚单层后加TET和 (或)PTX,连续培养48 h-四甲基偶氮唑盐(MTT)比色法分析细胞增殖情况,DAPI染色和流式细胞仪检测细胞凋亡情况-结果:TET和PTX单药或联合均呈剂量依赖性地抑制MKN-45细胞增殖,两药联合时IC50较两药单用时显著降低(P < 0.05)-DAPI染色和流式细胞仪显示TET和PTX联合用药组细胞凋亡率显著高于单药组(P < 0.05)-结论:TET联合PTX对胃癌细胞的增殖抑制及凋亡诱导有良好的协同效应-

    Abstract:

    Objective:To investigate the effects of tetrandrine (TET) in combination with paclitaxel (PTX) on the proliferation and apoptosis in human gastric cancer cell line MKN-45. Methods: MKN-45 cells were routinely cultured in RPMI-1640 medium supplemented with 10% heat-inactivated fetal calf serum. Subconfluent cell cultures were treated with either TET or PTX,or TET in combination with PTX for 48 h. Cell growth and proliferation of MKN-45 were analyzed with MTT assay. DAPI staining and flow cytometric analysis were used to detect the apoptosis of MKN-45. Results: Both TET and PTX alone or the combined treatment inhibited MKN-45 cell proliferation in a dose-dependent manner. The 50% inhibitory concentration (IC50) for TET and PTX were significantly decreased when MKN-45 cells were treated simultaneously with TET plus PTX compared with the two drugs applied alone(P < 0.05). DAPI staining and flow cytometric analysis showed that the apoptosis rates in the cells treated with TET and PTX were much greater than TET or PTX applied singly (P < 0.05). Conclusion: TET and PTX exerted a synergistic effect on the inhibition of proliferation and the induction of apoptosis in gastric cancer cells.

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郑锡凤,李晓林,何晓璞,徐 伟,唐 丹,张 皓,孙为豪.汉防己甲素联合紫杉醇对胃癌细胞增殖和凋亡的影响[J].南京医科大学学报(自然科学版),2012,(3):333-337

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  • 收稿日期:2011-11-07
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