牛磺熊脱氧胆酸对棕榈酸诱导的INS-1细胞凋亡的保护作用
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

江苏省自然科学基金面上项目(BK2011861);江苏省科技厅社会发展计划(BS2005066)


Protective effects of tauroursodeoxycholic acid on the apoptosis of INS-1 cells cultured by palmitate
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:探讨牛磺熊脱氧胆酸(tauroursodeoxycholic acid,TUDCA)对棕榈酸(palmitate)诱导的INS-1细胞凋亡的保护作用-方法:分别用不同浓度棕榈酸(0.25-0.50-1.00 mmol/L)-棕榈酸(0.50 mmol/L)加TUDCA(100 μmol/L)培养INS-1细胞24 h,用四甲基偶氮唑盐(MTT)法检测细胞活性,流式细胞术检测细胞早期凋亡,Western blot检测内质网应激相关蛋白GRP78的表达-结果:与对照组相比,棕榈酸组(浓度≥0.5 mmol/L)的INS-1细胞凋亡率显著上升(P < 0.05);加TUDCA培养24 h以后,与棕榈酸组相比,INS-1细胞凋亡率显著下降(P < 0.05)-此外,棕榈酸组(0.5 mmol/L)INS-1细胞内质网应激相关蛋白GRP78的表达显著上升(P < 0.05);加TUDCA培养24 h以后,与棕榈酸组相比,INS-1细胞GRP78蛋白表达显著下降(P < 0.05)-结论:TUDCA能够减少游离脂肪酸引起的INS-l细胞凋亡,对INS-1细胞发挥保护作用-而减少内质网应激蛋白的表达,缓解内质网应激可能是其作用机制之一-

    Abstract:

    Objective:To investigate the effect of tauroursodeoxycholic acid (TUDCA) on the apoptosis of INS-l cells cultured by palmitate in vitro. Methods: INS-1 cells were cultured in the presence of different concentrations of palmitate(0.25,0.50,1.00 mmol/L) or comlined with TUDCA(100 μmol/L) for 24 h. Cell viability was evaluated with MTT reduction conversion assay. The annexin-V/FITC cell death detection kit was used to monitor cell apoptosis. The expressions of endoplasmic reticulum(ER) stress marker GRP78 were detected by Western blotting. Results: The proliferation of INS-1 cells was inhibited,and the apoptosis was induced significantly by palmimte(concentration≥0.5 mmol/L,P < 0.05). The apoptosis rate was significantly decreased when INS-l cells were co-cultured with TUDCA(P < 0.05). Moreover,the expression of GRP78 was increased in INS-1 cells incubated with palmitate(P < 0.05),and it was repressed by TUDCA(P < 0.05). Conclusion: Our results suggest that TUDCA could protect INS-1 cells from palmitate-induced apoptosis,and it might work through repressing the expression of ER stress response protein GRP78 and alleviating ER stress.

    参考文献
    相似文献
    引证文献
引用本文

钟继娟,祝 群,缪 珩.牛磺熊脱氧胆酸对棕榈酸诱导的INS-1细胞凋亡的保护作用[J].南京医科大学学报(自然科学版),2012,(3):354-358

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2011-12-01
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期:
通知关闭
郑重声明