背根神经节微量注射7-硝基吲唑抑制炎症性疼痛
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江苏省自然科学基金重点研究专项(BK2011029)


Suppression of inflammatory pain by microinjecting 7-nitroindazole into dorsal root ganglion
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    摘要:

    目的:观察完全弗氏佐剂(complete Freund’s adjuvant,CFA)诱导炎症性疼痛的现象,并探究背根神经节(DRG)部位注射神经元型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)抑制剂7-硝基吲唑(7-nitroindazole,7-NI)对于炎症性疼痛是否有镇痛作用并简要探讨其镇痛机制-方法:小鼠足底注射10 -滋l CFA诱导炎症性疼痛动物模型,使用von Frey纤维丝采用up-and-down的方法测定小鼠的50%足底回缩阈值(50% paw withdrawal threshold,50%PWT)作为痛觉行为学的评价指标-在小鼠的背根神经节部位微量注射10 -滋g 7-NI观察50%PWT的变化情况-用免疫印迹的方法观察炎症相关的降钙素基因相关肽(calcitonin gene related peptide,CGRP)在CFA诱导模型后-造模后给予7-NI和nNOS基因敲除小鼠造模后的表达情况-结果:与DMSO组相比,7-NI组造模后2~12 h 50%PWT显著升高-观察到CGRP的表达量在CFA诱导后增加,而在给予7-NI和nNOS基因敲除组内CGRP的表达增加可以被逆转-结论:小鼠DRG部位注射7-NI对炎症性疼痛具有镇痛作用,且炎症性疼痛中CGRP表达量的增加可能是一氧化氮(nitric oxide,NO)在DRG内起疼痛敏感性作用的下游机制-

    Abstract:

    Objective:To observe the phenomenon of complete Freund’s adjuvant (CFA)-induced inflammatory pain and explore the analgesic effect of 7-nitroindazole(7-NI) on dorsal root ganglion(DRG),and briefly discuss its analgesic mechanism. Methods:Ten microliters of CFA were injected into the plantar surface of the hind paw of the mice to produce inflammatory pain models,von Frey filaments were used to determine 50% paw withdrawal threshold(PWT) adopting by up-and-down method which can be regarded as an evaluation criteria for the pain behavior test. The changes of 50% PWT were tested by microinjecting 10 -滋g 7-NI into DRG of the mice. Detect the expression of calcitonin gene related peptide (CGRP) in CFA-induced,7-NI treated and neuronal nitric oxide synthase(nNOS) knock-out models by Western blot. Results: Compared with DMSO group,50% PWT significantly increased in 7-NI group during 2~12 h after CFA inducing. The expression of CGRP increased after CFA injection,this effect could be reversed by 7-NI administration or nNOS knock-out. Conclusion: Microinjection of 7-NI into DRG could produce analgesia in inflammatory pain mice. Increased expression of CGRP might be the downstream mechanism resulted from NO mediated nociceptive effect in dorsal root ganglion.

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孙伟翔,徐海兵,朱东亚.背根神经节微量注射7-硝基吲唑抑制炎症性疼痛[J].南京医科大学学报(自然科学版),2012,(3):359-363

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  • 收稿日期:2011-10-26
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