D-松醇对慢性哮喘小鼠模型肺组织转化生长因子β1表达的影响
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南京医科大学科技发展基金重点资助项目(09NMUZ23)


Effect of D-pinitol on the TGF-β1 expression in a murine model of chronic asthma
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    目的:观察D-松醇对慢性哮喘小鼠模型转化生长因子(TGF-β1)的影响-方法:18只BALB/c小鼠按随机原则分成正常对照组-哮喘模型组-D-松醇干预组,每组6只,末次抗原激发24 h后,检测各组小鼠的肺功能,HE染色观察气道炎症变化;Masson三色染色观察气道纤维化的改变;real-time PCR观察肺组织TGF-β1 mRNA的表达;采用ELISA观察支气管肺泡灌洗液中TGF-β1的表达-结果:哮喘模型组小鼠气道炎症-气道高反应性和气道重塑明显加重,而D-松醇能显著抑制慢性哮喘模型小鼠的气道炎症和气道高反应性,且D-松醇干预后哮喘模型小鼠肺组织TGF-β1 mRNA和肺泡灌洗液中TGF-β1表达也显著降低-结论:D-松醇能抑制慢性哮喘模型小鼠的气道重塑,其机制可能通过抑制肺组织TGF-β1的表达而实现-

    Abstract:

    Objective:To observe the effect of D-pinitol on the expression of transforming growth factor-β1(TGF-β1) in a murine model of chronic asthma. Methods: Eighteen BALB/c mice were randomly divided into 3 groups,namely control group,OVA group and D-pinitol group. Pulmonary function was measured to evaluate the resistance of expiration 24 hours after the last OVA challenge,hematoxylin and eosin(HE) staining was used to assess the inflammatory cell infiltrates,and Masson’s trichrome to determine airway fibrosis. The expression of TGF-β1 mRNA in lung was measured by real-time PCR. The concentration of TGF-β1 in bronchoalveolar lavage fluid (BALF) was measured by enzyme-linked immunoabsorbent assay (ELISA). Results: All the parameters associated with airway inflammation,airway hyperresponsiveness (AHR) and remodeling were increased in the OVA group,and decreased by treatment of D-pinitol(P < 0.05). Treatment with D-pinitol significantly inhibited the levels of TGF-β1 in the lung tissue and in the BALF(P < 0.05). Conclusion: These data demonstrated that D-pinitol could suppress the progression of airway remodeling in a murine model of chronic asthma. Partly,the effect might be due to inhibition of the expression of TGF-β1.

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顾晓燕,王毓薇,蒋德升,杜 强. D-松醇对慢性哮喘小鼠模型肺组织转化生长因子β1表达的影响[J].南京医科大学学报(自然科学版),2012,(3):369-373

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  • 收稿日期:2011-03-28
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