5-Fu减轻急性MODS模型全身性损伤的实验研究
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南京市医学科技发展项目(YKK08085)


Experimental study of 5-Fu to reduce systemic injury in acute model of MODS
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    摘要:

    目的:观察单次剂量5-氟尿嘧啶(5-Fu)对多器官功能障碍大鼠多脏器功能及其存活时间的影响-方法:应用多发性骨折+烫伤+绿脓杆菌感染方法制造SD大鼠急性多器官功能障碍模型-动物被随机分成多器官功能障碍综合征模型组(MODS模型组,n = 36)和模型+5-Fu(50 mg/kg)处理组(5-Fu组,n = 26),每组分4个亚组-分别于模型复制成功后24-48-72-96 h处死大鼠,取血进行外周血白细胞计数,检测白细胞功能-肝肾功能-心肌酶谱,同时观察动物的死亡率-结果:与MODS模型组动物比较,实验早中期5-Fu抑制了动物外周血白细胞计数增加-5-Fu对白细胞的呼吸爆发无影响;但能下调白细胞CD11b表达-实验期间,5-Fu处理组大鼠死亡率明显降低,血清丙氨酸氨基转移酶和门冬氨酸氨基转移酶-磷酸肌酸激酶和肌酸激酶同工酶活性-尿素氮等指标改善不显著,但实验组血糖值波动平缓,而对照组血糖进行性上升-结论:单次给予5-Fu能够对多器官功能障碍模型大鼠产生器官保护作用-

    Abstract:

    Objective:To observe the effects of a single dose of 5-fluorouracil(5-Fu) on multiple organ functions and the survival time of rats with multiple organ dysfunction syndrome (MODS). Methods: The study adopted the methods of multiple fractures + scald + Pseudomonas aeruginosa infection to produce the model of SD rats with acute MODS. The rats were randomly divided into MODS model group(n = 36) and treatment group (MODS model+5-Fu,n = 26),each being divided into four subgroups. The rats were sacrificed 24,48,72 or 96 h after the models were built successfully,blood samples were collected to examined the leukocyte numbers of peripheral blood,the leukocyte function,liver and kidney function and myocardial enzymes. Rats’ mortality rate was observed as well. Results: Compared with MODS model group,5-Fu restrained the leukocyte numbers of peripheral blood in the early and middle stages. 5-Fu had no effect on leukocyte respiratory burst but reduced the CD11b expression. During the experiment,rats’ mortality rate of 5-Fu treatment group decreased significantly. There were no differences in serum activity of alanine aminotransferase,aspartate aminotransferase,creatine kinase,creatine kinase isoenzyme,and no differences in blood urea nitrogen between the two groups. The blood glucose level were improved at different stages of 5-Fu group,but increased progressively in contrast group. Conclusion: A single administration of 5-Fu may protect rats with MODS against systemic injury.

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卞建民,江 强,章世海.5-Fu减轻急性MODS模型全身性损伤的实验研究[J].南京医科大学学报(自然科学版),2012,(3):374-377

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  • 收稿日期:2011-09-06
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