原发性高血压患者单核细胞TRPC3的表达水平与细胞因子的关系
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:


Relations between TRPC3 expression and cytokines in monocytes from patients with essential hypertension
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:探讨原发性高血压(essential hypertension,EH)患者单核细胞中经典瞬时受体电位通道3(transient receptor potential canonical channel 3,TRPC3)转录水平与单核细胞中白细胞介素1β(interleukin-1β,IL-1β)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的转录水平以及外周血单核细胞趋化蛋白1(monocyte chemotactic protein 1,MCP-1)浓度的关系-方法:抽取20例原发性高血压患者和20例血压正常的健康体检者的静脉血,分离获得单核细胞,运用实时定量聚合酶链反应法(real-time PCR)分别检测外周血单核细胞中TRPC3-TNF-α和IL-1β的mRNA,用酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)检测外周血MCP-1浓度-结果:与血压正常对照组比较,原发性高血压组单核细胞中TRPC3-TNF-α和IL-1β的mRNA表达升高,MCP-1浓度增高,具有统计学差异(P < 0.05)-单核细胞中TRPC3的mRNA水平与TNF-α和IL-1β的mRNA水平以及MCP-1浓度呈现正相关-结论:原发性高血压作为一个慢性炎症过程,单核细胞中TRPC3表达与细胞因子水平相关,TRPC3表达上调可被认为是单核细胞活化的指标之一-

    Abstract:

    Objective:To investigate the relationship of transient receptor potential canonical channel 3 (TRPC3) transcription and mRNA levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in monocytes,and monocyte chemoattractant protein 1 (MCP-1) level in peripherial blood of patients with essential hypertension (EH). Methods: The levels of TRPC3,TNF-α and IL-1β mRNA in monocytes of 20 patients with essential hypertension and 20 normotensive controls were analyzed by real-time PCR,the concentration of MCP-1 was measured by enzyme linked immunosorbent assay (ELISA). Results: The elevated TRPC3,TNF-α,IL-1β mRNA levels in monocytes and MCP-1 protein in peripherial blood were observed in patients with essential hypertension. There were significant differences between the two groups(P < 0.05). The level of TRPC3 mRNA was positively correlated with the levels of TNF-α,IL-1β mRNA and MCP-1 protein. Conclusion: Essential hypertension is condsidered as a chronic inflammation process. The expression of TRPC3 in monocytes is related to cytokines. Overexpression of TRPC3 may be regarded as one of indicators of activations of monocytes.

    参考文献
    相似文献
    引证文献
引用本文

马前军,孙康云,程志坚,曾元英,钱丽娟.原发性高血压患者单核细胞TRPC3的表达水平与细胞因子的关系[J].南京医科大学学报(自然科学版),2012,(3):382-385

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2011-04-23
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期:
通知关闭
郑重声明