非梗阻性无精子症的精母细胞同源染色体联会分析
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国家杰出青年科学基金(308250381)


Analysis of homologous synapsis in spermatocytes of non-obstructive azoospermics
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    摘要:

    目的:探讨Y微缺失与同源染色体联会异常在精子发生障碍中的意义-方法:选择无Y微缺失的单纯精子发生障碍患者3例-合并有Y微缺失和AZFc缺失的精子发生障碍患者3例-无Y微缺失的精子发生正常者(3例)为对照组;利用免疫荧光技术比较3组睾丸组织中精母细胞的同源染色体联会情况,包括联会复合体(synaptonemal complexes,SC)上gap和split出现的数目-结果:SC中出现gap的频率在对照组为10%-单纯精子发生障碍组为22%-合并有Y微缺失的精子发生障碍组为25%-两个精子发生障碍组与对照组相比均有显著性差异(P < 0.05),两个精子发生障碍组之间无统计学差异-SC中出现split的频率对照组为1%-单纯精子发生障碍组为13%-合并有Y微缺失的精子发生障碍组为15%-两个精子发生障碍组与对照组相比均有显著性差异(P < 0.01),两个精子发生障碍组之间无统计学差异-结论:精母细胞中同源染色体联会异常是影响精子发生的重要机制之一,Y微缺失的睾丸组织精母细胞中同源染色体联会异常有加重的趋势-

    Abstract:

    Objective:To investigate the influence of Y chromosome microdeletion and homologous synapsis abnormalities in spermatocytes of male spermatogenesis failure. Methods: Nine cases were recruited in this study,including controls (3 cases),with normal spermatogenesis in their testes;spermatogenesis failure group (3 cases),with spermatogenesis arrest in their testes and without Y microdeletion;and spermatogenesis failure with Y microdeletion group (3 cases). Immunofluorescent staining technology was used to analyze synaptonemal complexes (SC) of spermatocytes. Results: There were 10%,22%,25% gaps presented in testes of controls,spermatogenesis failure group and spermatogenesis failure with Y microdeletion group,respectively. There were significant differences between two spermatogenesis failure groups and controls (10% vs. 22%,P = 0.012;10% vs. 25%,P = 0.014). However,there was no significant difference between two spermatogenesis failure groups. There were 1%,13%,15% splits presented in testes of controls,spermatogenesis failure group and spermatogenesis failure with Y microdeletion group,respectively. There were significant differences between two spermatogenesis failure groups and controls (1% vs. 13%,P = 0.000;1% vs. 15%,P = 0.000). However,there was also no significant difference between two spermatogenesis failure groups. Conclusion: Homologous synapsis abnormalities may play an important factor in spermatogenesis failure. Furthermore,homologous synapsis abnormalities would be more frequent in infertile men with Y chromosome microdeletion.

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张秋芳,黄 翔,闫丽盈,乔 杰.非梗阻性无精子症的精母细胞同源染色体联会分析[J].南京医科大学学报(自然科学版),2012,(3):399-402

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  • 收稿日期:2012-01-10
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