Objective: To investigate the serum level of S100A12 in coronary heart disease (CHD) patients and approach the effects of S100A12 in diagnosis of CHD. Methods: One hundred and twenty-eight patients who underwent CHD and sixty-one patients with normal coronary artery CTA or coronary angiography were selected in the study. Serum level of S100A12 was measured by ELISA assay. The relationship between serum level of S100A12 and the pathogenetic conditions of CHD were analyzed by logistic regression. Results: The serum level of S100A12[(43.57 ± 10.30) ng/ml] in CHD group was significantly higher than those in serum of non-CHD group[(25.63 ± 7.42) ng/ml](P < 0.001),and positively related with the coronary stenosis Gensini severity score (r = 0.391,P < 0.001). Further logistic regression analysis indicated that after ruling out the other risk factors,such as age,male,BMI overweight,high blood pressure,diabetes,and blood lipid abnormalities,S100A12 also had significant correlation with CHD (OR = 2.67,P < 0.001). Conclusion: The serum level of S100A12 may be a potential biological indicator to predict the risk for CHD.