Objective:To analyze the concordance and correlation of the detection results of HBsAg,anti-HBs and HBeAg,which were measured by Roche Cobas e601 and Abott Architect i2000,respectively. Methods:The serum samples,including 119 for HBsAg detection,140 for anti-HBs detection and 197 for HBeAg detection,were differently measured by these two chemiluminescence immunoassay(CLIA) systems. Results:The concordance of qualitative results for HBsAg,anti-HBs and HBeAg were 99.1%,95.7% and 90.9% between these two CLIA systems,and the correlation coefficient of quantitative results were 0.566,0.863 and 0.964. But the anti-HBs concentration measured by Abott Architect i 2000 was significantly higher than that of Roche Cobas e 601 system. Conclusion:Qualitative detection results of HBV markers in serum measured by these two systems have good concordance,but anti-HBs concentrations detected were significant different between these two systems.