Objective:To investigate the effects of BVT.2733 on the expression of Visfatin and insulin resistance in the diet-induced obese(DIO) mice model. Methods:The C57BL/6J mice were randomly divided into the normal diet group and high-fat diet (HFD) group. After 20 weeks,the obese mice were randomly divided into obese control group and BVT.2733 treatment group. And they were orally administered placebo and BVT.2733,respectively,for two weeks. The levels of plasma glucose and serum insulin were measured by biochemistry technology and radioimmunity. The visfatin mRNA expression in adipose tissue were analyzed by real-time quantitative PCR. And the morphology changes of adipocyte were also observed. Results:Comparing to normal controls,the weight,plasma glucose and serum insulin levels increased in HFD group(P < 0.05),and the size of adipocyte in HFD group was big. Comparing to obese controls,in BVT.2733 treatment group,the serum insulin significantly decreased(P < 0.01) and the adipocyte sizes reduced,while the mRNA expression of Visfatin down-regulated(P < 0.05). Conclusion:BVT.2733 could reduce body weight significantly,ameliorate insulin resistance,and down-regulate the expression of Visfatin.