血小板环氧化酶及P-选择素的检测及其在冠心病患者中的表达
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国家自然科学基金(81170181);江苏省人民医院创新团队基金(2011)


Determination of platelet cyclooxygenases and P-selectin in patients with coronary artery disease
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    摘要:

    目的:建立用流式细胞术测定富血小板血浆(platelet-rich plasma,PRP)中血小板环氧化酶-1(cyclooxygenase-1,COX-1)和环氧化酶-2(cyclooxygenase-2,COX-2)及P-选择素表达的方法;检测在无抗血小板药物干预的状况下中国汉族冠心病患者血小板COX-1-COX-2及P-选择素的表达情况-方法:入选拟行冠状动脉搭桥术-停用阿司匹林及氯吡格雷7 d以上的冠心病患者20例,采集空腹静脉血,经预处理及荧光标记抗体孵育后用流式细胞仪检测血小板COX-1-COX-2及P-选择素的表达情况-结果:在无抗血小板药物干预的状况下,中国汉族冠心病患者血小板COX-1-COX-2阳性表达比例分别为(39.67 ± 9.20)%和(4.11 ± 2.74)%;生理盐水刺激后血小板膜表面P-选择素的阳性表达率为(13.56 ± 8.07)%,花生四烯酸刺激后的阳性表达率为(24.08 ± 13.49)%,血小板膜表面P-选择素的实际阳性表达率为(10.27 ± 7.40)%-结论:流式细胞术可有效测定血小板COX-1-COX-2和P-选择素的表达,冠心病患者血小板COX-1-COX-2和P-选择素的表达在中国汉族人种与高加索人种间可能存在差异-

    Abstract:

    Objective:To establish a method of detecting cyclooxygenase (COX)-1,COX-2 and P-selectin in platelet-rich plasma (PRP) by flow cytometry(FCM),and to detect the expressions of COX-1,COX-2 and P-selectin in PRP from coronary artery disease (CAD) patients without using anti-platelet agents. Methods:Twenty CAD patients were recruited,who were going to receive coronary artery bypass graft(CABG) and had withdrawn aspirin and clopidogrel for at least 7 days. After overnight fasting,the patients’ venous blood was drawn and processed,then the PRP was incubated with fluorescence labeled antibody,and COX-1,COX-2 and P-selectin were detected by FCM. Results:The positive percent of COX-1,COX-2 in PRP of the enrolled 20 patients were (39.67 ± 9.20)% and (4.11 ± 2.74)%,respectively,and the positive percent of P-selectin in platelet was (13.56 ± 8.07)% after incubated with saline and(24.08 ± 13.49)% after incubated with arachidonic acid,the real positive percent of P-selectin in platelet was(10.27 ± 7.40)%. Conclusion:FCM can effectively detect the platelet expressions of COX-1,COX-2 and P-selectin. There would be ethnic differences between Chinese Han and Caucasian regarding the platelet expressions of COX-1,COX-2 and P-selectin in CAD patients.

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龚晓璇,张锦英,张 秋,朱甜甜,王晓伟,王学忠,殷咏梅,李春坚,杨志健.血小板环氧化酶及P-选择素的检测及其在冠心病患者中的表达[J].南京医科大学学报(自然科学版),2012,(7):948-951

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  • 收稿日期:2012-03-27
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