儿童支原体肺炎血清IL-8?TNF-α和hs-CRP的检测意义
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南京医科大学科技发展基金(2010NJMU049)


Determination of serum levels of IL-8,TNF-α and hs-CRP in children with mycoplasma pneumoniae pneumonia
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    摘要:

    目的:检测不同程度支原体肺炎(mycoplasma pneumoniae pneumonia,MPP)患儿血清白细胞介素-8(interleukin-8,IL-8)-肿瘤坏死因子α ( tumor necrosis factor alpha,TNF-α)和超敏C反应蛋白(high-sensitivity C-reactive protein,hs-CRP)的动态变化,探讨其在MPP发病机制中的作用及临床意义-方法:收集2009年1月~2011年12月南京医科大学附属南京医院儿科住院诊断明确的MPP患儿60例,其中重症30例,以同期30例体检的儿童作为正常对照组-采用酶联免疫吸附实验(enzyme-linked immunosorbent assay,ELISA )检测患儿血清IL-8和TNF-α的质量浓度,免疫比浊法测定血清hs-CRP的浓度,用单因素方差分析法比较患儿血清中IL-8-TNF-α和hs-CRP在不同病期和轻-重症中的表达水平-结果: MPP患儿血清IL-8-TNF-α水平在急性期和恢复期均明显高于正常对照组(P < 0.01),且重症组显著高于轻症组(P < 0.01);急性期重症组hs-CRP水平高于轻症组及正常对照组(P < 0.01),而在恢复期,hs-CRP水平下降,MPP患儿与正常对照组比较,差异无统计学意义(P > 0.05)-结论:IL-8-TNF-α和hs-CRP在小儿MPP的发病过程中发挥重要作用,动态监测患者血清IL-8-TNF-α和hs-CRP水平变化对MPP的病情监测及治疗有重要的指导意义-

    Abstract:

    Objective:To investigate the changes of inflammatory cytokines interleukin-8 (IL-8),tumor necrosis factor alpha(TNF-α)and high-sensitivity C-reactive protein(hs-CRP) in children in different phases of mycoplasma pneumoniae pneumonia(MPP). Methods:Sixty children with MPP and thirty healthy children were recruited from January 2009 to December 2011 in Nanjing Hospital Affiliated to NJMU. The mass concentration of the IL-8 and TNF-α was measured by enzyme linked immunosorbent assay(ELISA),the concentration of hs-CRP was measured by immune turbidimetry. The serum levels of the IL-8,TNF-α and hs-CRP were compared in different phases and stages with one-way analysis of variance(one-way ANOVA). Results:The serum levels of the IL-8 and TNF-α in the children with MPP in both acute stage and convalescence stage were significantly higher than normal control(P < 0.01). The levels of IL-8 and TNF-α in the severe cases were higher than those in the mild cases(P < 0.01). The level of the hs-CRP in the severe cases was higher than those in the mild cases and normal control in acute stage(P < 0.01). While there was no significant difference in the level of hs-CRP among three groups in convalescence stage (P > 0.05). Conclusion:Inflammatory cytokines of IL-8,TNF-α and hs-CRP play important roles in the pathogenesis of MPP. They can be used as monitoring indicators to reflect the severity of pneumonia.

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王晓花,张 莉,于 莹.儿童支原体肺炎血清IL-8?TNF-α和hs-CRP的检测意义[J].南京医科大学学报(自然科学版),2012,(7):969-972

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  • 收稿日期:2012-03-12
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