Objective:To analyze the sensitivity and specificity of adenosine stress myocardial perfusion imaging for the diagnosis of coronary artery disease(CAD) and to observe the adverse reaction of adenosine burden test. Methods:A total of 85 inpatients were included in this study. SPECT myocardial imaging acquisition was obtained 1 hour after adenosine infusion. Rest myocardial perfusion imaging would be performed next day regularly. Coronary angiography was performed in 67 patients within one month of myocardial imaging. Results:① Total 85 cases[(66 ± 9) years old,46 men,39 women]were included in this study. In the 52 cases of CAD patients confirmed by coronary angiography,46 patients have positive adenosine 99mTc-MIBI myocardial perfusion SPECT. Of 15 cases without CAD,11 cases have negative adenosine myocardial perfusion tomographic imaging. The sensitivity and specificity of adenosine myocardial perfusion tomographic imaging for the diagnosis of CAD were 88.5% and 73.3%. ② In the 85 patients,8.3% patients had no symptom,91.7% had varied kinds of adverse reaction,consisted of chest complaint(83.3%),skin aestus(23.1%),dizziness(20.5%),palpitation(12.8%),short breath(7.7%),sweating(7.7%) and so on. At the end of 5 minutes of stopping adenosine infusion,all adverse reaction disappeared,heart rate and blood pressure returned to basal level. Conclusion:Adenosine stress myocardial perfusion tomographic imaging is an useful and safty non-interventional method for detecting coronary artery disease.