人特异性蛋白激酶-15基因在增生牙龈组织中表达的研究
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国家自然科学基金(81170962);江苏省自然科学基金(BK2011763);江苏省高校优势学科建设工程资助


The expression of human-specific protein kinase-15 in the hyperplastic gingival tissues
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    摘要:

    目的:检测细胞增殖相关基因人特异性蛋白激酶-15(specific protein kinase 15,NYD-SP15)基因在增生牙龈及正常牙龈组织中的表达,初步探讨该基因与牙龈增生发生的关系-方法:应用RT-PCR及免疫组化法检测27例正常牙龈组织(取自外伤冠折后行牙冠延长术中切除的牙龈组织)及40例牙龈增生患者(以增生表现为主的慢性牙龈炎12例-药物性牙龈增生13例-牙龈瘤15例)牙龈切除术中切除的增生牙龈组织标本中NYD-SP15 mRNA及蛋白的表达-结果:增生牙龈组织中NYD-SP15的mRNA水平显著高于正常牙龈组织(P < 0.05)-免疫组化结果显示,在正常牙龈组织中该基因高表达于胞浆,在增生牙龈组织中高表达于胞核-结论:NYD-SP15表达量异常以及定位差异在人牙龈增生发生发展过程中起到一定的作用,但该基因高表达与牙龈增生发生的相关机制尚待进一步研究-

    Abstract:

    Objective:To detect the human-specific protein kinase-15(NYD-SP15) gene expression in normal gingival tissue and hyperplastic gingival tissues,observe the expression of NYD-SP15 protein in gingival tissues,and to explore the relationship of NYD-SP15 and gingival hyperplasia initially. Methods:Reverse transcription-polymerase chain reaction(RT-PCR) and immunohistochemical method were applicated to detect the expression of NYD-SP15 gene in gingival tissues of 27 cases of normal people and 40 cases of gingival hyperplasia patients. The normal gingival tissues were the excision of gingival tissue during crown lengthening surgery (the teeth were traumatic crown fractures),recorded as normal control group. The hyperplasia samples were the excision of gingival tissue during gingivectomy,recorded as hyperplasia group. The half of all specimens were removed and stored in nitrogen liquid immediately. The remaining half of the tissues with formalin,embedded in paraffin storage. Staining positive cells were counted under the microscope and classificated by positive degree. The data of the experiment was analyzed by Stata 9.2(StataCorporation College Station,TX) software. Results:The expression of NYD-SP15 mRNA in tissues of hyperplasia group was significantly higher than that of control group(P < 0.05). The immunohistochemistry results showed that the NYD-SP15 protein was highly expression in the cytoplasm in normal gingival tissue. But,in the hyplasia gingival tissues,the expression of NYD-SP15 protein was highly in the nucleus. Conclusion:The level of NYD-SP15 expression and location in the gingival tissues may be associated with the gingival hyperplasia. However,the relationship between them remains to be further studied.

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梅幼敏,宦 泓,周 艳,谢海燕,张汝阳,徐 艳.人特异性蛋白激酶-15基因在增生牙龈组织中表达的研究[J].南京医科大学学报(自然科学版),2012,(10):1372-1375

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  • 收稿日期:2012-04-19
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