硫化氢对H2O2所致大鼠胸主动脉损伤的保护作用
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南京医科大学科技发展基金重点项目(2011 NJMU264)


Hydrogen sulfide has protective effect against H2O2-induced injury in isolated thoracic aorta of rats
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    目的:探讨硫化氢(hydrogen sulfide,H2S)对过氧化氢(hydrogen peroxide,H2O2)引起的大鼠离体胸主动脉损伤的保护作用和机制-方法:分离正常大鼠胸主动脉,制备主动脉环,利用300 μmol/L H2O2建立大鼠胸主动脉环氧化应激损伤模型-25-50-100 μmol/L的硫氢化钠(sodium hydrosulfide,NaHS)预处理后再用H2O2损伤大鼠胸主动脉环,应用血管功能检测张力仪比较各组舒张功能,并采用二氢乙啶(dihydroethidium,DHE)荧光染色法测定活性氧自由基(reactive oxygen species,ROS)的生成-Western blot法检测大鼠胸主动脉环中ERK1/2和磷酸化ERK1/2(p-ERK1/2)蛋白的表达-结果:300 μmol/L的H2O2可引起大鼠胸主动脉环内皮依赖性舒张功能受损,50-100 μmol/L的NaHS预处理后该损伤得到明显改善,使H2O2诱发的ROS亦显著减少-同时,H2O2可激活大鼠胸主动脉组织中的MAPK/ERK通路,使其磷酸化水平增加,而给予50-100 μmol/L的NaHS预处理后可抑制该作用-结论:硫化氢对H2O2致大鼠胸主动脉氧化应激性损伤有保护作用,其机制可能与调节ERK信号通路有关-

    Abstract:

    Objective:To investigate the protective effect and mechanism of hydrogen sulfide (H2S) on hydrogen peroxide(H2O2)-induced injury in isolated thoracic aorta of rats. Methods:Thoracic aortic rings were isolated from normal Sprague-Dawley rats,subsequently exposed to 300 μmol/L H2O2 to establish an oxidative stress model. The thoracic aortic rings were pre-treated with 25 μmol/L,50 μmol/L or 100 μmol/L of NaHS followed by incubation with 300 μmol/L H2O2. The endothelium-dependent vasorelaxant function of thoracic aortic rings was measured by a pressure transducer coupled to a Labchart V6 System. Production of ROS was evaluated using DHE fluorescent staining assay. The expressions of protein MAPK/ERK1/2 and p-ERK1/2 in thoracic aortic were determined by Western blot analysis. Results:H2O2 of 300 μmol/L can decrease vasorelaxant function in rats’ thoracic aortic rings. Pre-treated with 50 μmol/L or 100 μmol/L of NaHS can attenuate the damage and decrease the production of ROS caused by H2O2. H2O2 also activated MAPK/ERK pathway in the isolated rats’ thoracic aorta,which could be inhibited by NaHS of 50 μmol/L or 100 μmol/L. Conclusion:H2S may have protective effect against H2O2-induced injury through ERK signal pathway in isolated thoracic aorta of rats.

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陈丽灵,唐 燕,刘 振,孟国梁,白文莉,李 莎,谢利平,季 勇.硫化氢对H2O2所致大鼠胸主动脉损伤的保护作用[J].南京医科大学学报(自然科学版),2012,(11):1499-1504

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  • 收稿日期:2012-08-20
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