齐墩果酸对损伤血管内皮细胞的保护作用及其机制探讨
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Protective effects and mechanisms of oleanolic acid on damaged human umbilical vein endothelial cells
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    摘要:

    目的:探讨齐墩果酸(oleanic acid,OA)对过氧化氢(hydrogen peroxide,H2O2)诱导人血管内皮细胞(human umbilical vein endothelial cells,HUVECs)损伤的保护作用及其机制-方法:培养HUVECs,分为正常组-H2O2模型组-H2O2+OA (0.25 -滋mol/L)组-H2O2+OA (0.50 -滋mol/L)组-H2O2+OA (1.00 -滋mol/L)组-H2O2+维生素C(1.50 mmol/L)组-各组培养24 h后,通过MTT法检测细胞增殖能力,酶生化法测定上清液中的乳酸脱氢酶(lactate dehydrogenase,LDH)和一氧化氮(nitric oxide,NO)含量,酶生化法测定细胞裂解液中的丙二醛(malondialdehyde,MDA)含量-谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)和超氧化物歧化酶(superoxide dismutase,SOD)活力,流式细胞仪测细胞内活性氧(reactive oxygen species,ROS)和细胞凋亡率-结果:OA能够恢复H2O2损伤的细胞活力,减轻H2O2诱导的LDH释放,减少H2O2诱导的MDA生成,恢复H2O2损伤的GSH-Px和SOD活力,清除H2O2诱导的ROS,减轻H2O2诱导的细胞凋亡,提高HUVECs细胞NO的生成-结论:OA对损伤血管内皮细胞有保护作用,这与OA能够启动HUVECs细胞内抗氧化防御机制,清除细胞内的ROS,提高HUVECs细胞NO的生成,抑制H2O2诱导的HUVECs细胞凋亡有关-

    Abstract:

    Objective:To study the protective effects and mechanisms of oleanic acid on damaged human umbilical vein endothelial cells (HUVECs) induced by hydrogen peroxide (H2O2). Methods:HUVECs were divided into control group,H2O2 group(200 mol/L H2O2 treatment),H2O2 of 200 mol/L combined with OA of 0.25 mol/L treatment group,H2O2 of 200 mol/L combined with OA of 0.50 mol/L treatment group,H2O2 of 200 mol/L combined with OA of 1.00 mol/L treatment group,H2O2 of 200 mol/L combined with vitamin C of 1.50 mmol/L treatment group. Cell viability was determined by MTT method. Lactate dehydrogenase(LDH) and nitric oxide (NO) content in the supernatant were determined by biochemical method. The activity of malondialdehyde(MDA),glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in the cell lysate was determined by biochemical method. Intracellular reactive oxygen species (ROS) and cell apoptosis were determined by flow cytometry. Results:OA restored the H2O2-injuried cell viability,reduced the H2O2-induced LDH release,and reduced H2O2-induced MDA formation,recovered H2O2-injuried GSH-Px and SOD activity,removed H2O2-induced ROS,reduced the H2O2-induced apoptosis,and enhanced the NO production in HUVECs. Conclusion:OA has a protective effect on HUVECs,which is related to initiating the antioxidant defense,getting rid of ROS,inhibiting H2O2-induced apoptosis,and improving NO production in HUVECs.

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魏海珍,张秀峰.齐墩果酸对损伤血管内皮细胞的保护作用及其机制探讨[J].南京医科大学学报(自然科学版),2012,(11):1505-1510

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  • 收稿日期:2012-09-04
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