急性心肌梗死患者血管紧张素Ⅱ水平对外周血内皮祖细胞的影响
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常州市卫生局重大课题(ZD201012)


Effect of angiotensin Ⅱ concentration on peripheral blood endothelial progenitor cells in acute myocardial infraction
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    目的:急性心肌梗死(acute myocardial infraction,AMI)患者外周血中血管紧张素Ⅱ(angiotensin Ⅱ,Ang-Ⅱ)水平对内皮祖细胞(endothelial progenitor cells,EPCs)的数目变化的影响-方法:入选的28例AMI患者(实验组)和30例冠状动脉造影阴性患者(对照组),用ELISA法检测AMI患者急性期-恢复期以及对照组血清中Ang-Ⅱ-基质细胞衍生因子-1α(stromal cell-derived factor-1α,SDF-1α)的水平,同时用密度梯度离心法获得单个核细胞,用CD34-VEGF和CD133抗体标定EPCs,用流式细胞仪检测外周血中EPCs占单个核细胞的比率-结果:AMI患者急性期Ang-Ⅱ水平(90.40 ± 6.39)pg/ml明显高于恢复期 (72.57 ± 2.42)pg/ml和对照组(69.14 ± 3.73)pg/ml (P = 0.002 4),同时AMI患者急性期EPCs占外周血单个核细胞的比率为为(0.043 ± 0.004)%,明显高于恢复期(0.024 ± 0.010)% 和对照组(0.012 ± 0.001)%(P < 0.000 1),但AMI患者急性期SDF-1α的水平(171.1 ± 35.81) pg/ml同恢复期(130.30 ± 28.84) pg/ml及对照组(116.50 ± 29.19) pg/ml之间无明显差异(P = 0.370 3)-在AMI患者急性期外周血中EPCs和Ang-Ⅱ水平呈负相关,而与SDF-1α水平无明显相关性-结论:AMI患者急性期循环中的Ang-Ⅱ水平及EPCs数量均升高,但是Ang-Ⅱ抑制了EPCs的数量-

    Abstract:

    Objective:To investigate the effect of angiotensin Ⅱ(Ang-Ⅱ) on numerous of endothelial progenitor cells(EPCs) in acute myocardial infraction(AMI). Methods:Twenty-eight patients with AMI and 30 controls with negative results of coronary artery angiography were selected in the study. The concentration of Ang-Ⅱ and stromal cell-derived factor-1α(SDF-1α) in different periods of patients was determined with immunosorbent assay(ELISA). Circulating EPCs were stained with anti-CD34,VEGF and CD133 antibodies,and then the ratio of EPCs to plasma mononuclear cells was analyzed by three-colour flow cytometry. Results:The concentration of Ang-Ⅱ in acute stage of AMI patients was(90.40 ± 6.39) pg/ml and higher than that of AMI patients in recovery stage[(72.57 ± 2.42)pg/ml] and control group[(69.14 ± 3.73)pg/ml](P = 0.002 4). But the concentration of SDF-1α of AMI patients in acute stage was (171.10 ± 35.81)pg/ml and had not significant difference with that of AMI patients in recovery stage[(130.30 ± 28.84)pg/ml] and control group[(116.50 ± 29.19)pg/ml](P = 0.370 3). Circulating EPCs were significantly increased in acute stage of AMI patients[(0.043 ± 0.004)%]compared with the recovery stage of AMI patients[(0.024 ± 0.010)%]and control group[(0.012 ± 0.001)%](P < 0.000 1). In acute stage of AMI patients,circulating EPCs had negative correlation with the concentration of Ang-Ⅱ (r = -0.801,P < 0.001),but had no correlation with the concentration of SDF-1α(r = -0.076,P = 0.702). Conclusion:Quantity of EPCs and concentration of Ang-Ⅱ increased in acute stage of AMI patients,but Ang-Ⅱ could reduce the quantity of EPCs.

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朱傲霜,丁志坚,卢绪章,夏 蕾,李海燕.急性心肌梗死患者血管紧张素Ⅱ水平对外周血内皮祖细胞的影响[J].南京医科大学学报(自然科学版),2012,(11):1565-1569

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  • 收稿日期:2012-07-17
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