维生素D受体mRNA及其蛋白在不同体质指数人群皮下和大网膜脂肪组织中的表达差异
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南京医科大学科技发展基金(2010NJMUZ50)


The mRNA and protein expression differences of vitamin D receptor in subcutaneous and omental adipose tissues of people with different BMI
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    目的:观察正常-超重和肥胖3组人群皮下和大网膜脂肪组织中维生素D受体(vitamin D receptor,VDR)mRNA及其蛋白表达水平的差异-方法:用半定量RT-PCR方法检测20例正常组-35例超重组-27例肥胖组人群的腹部皮下和大网膜脂肪组织VDR基因的mRNA表达水平;用Western blot方法检测VDR的蛋白表达水平-结果:在皮下脂肪组织中,肥胖组VDR mRNA和蛋白表达水平明显低于正常组和超重组(P < 0.05);在大网膜脂肪组织中,超重组VDR mRNA及其蛋白表达增高(P < 0.05),肥胖组VDR mRNA及其蛋白表达与超重组相比降低(P < 0.05)-结论:肥胖患者脂肪组织中VDR表达水平降低,可能是临床上补充维生素D干预肥胖达不到预期效果的机制之一-

    Abstract:

    Objective:To investigate the mRNA and protein expression differences of vitamin D receptor(VDR) in subcutaneous and omental adipose tissue of people with different BMI. Methods:Twenty people of normal BMI,35 people of overweight and 27 people of obesity were selected. The mRNA transcription levels of VDR were assayed by RT-PCR,and the VDR protein expression was measured by Western blot. Results:① In subcutaneous obesity tissue,mRNA transcription and protein expression of VDR was lower in obesity group than in the normal or overweight group(P < 0.05);② In omental adipose tissue,mRNA transcription and protein expression of VDR was highest in overweight group among 3 groups(P < 0.05). Compared with the overweight group,mRNA transcription and protein expression of VDR was lower in obesity group(P < 0.05). Conclusion:The VDR expression decreased in the obesity group,which may be one of the mechanisms of the poor clinical outcome of vitamin D intervention therapy.

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徐家蓉,张力翔,缪 珩.维生素D受体mRNA及其蛋白在不同体质指数人群皮下和大网膜脂肪组织中的表达差异[J].南京医科大学学报(自然科学版),2012,(11):1575-1578

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  • 收稿日期:2012-07-23
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