Objective:To investigate the expression of Sox2 in human bladder cancer and evaluate the effect of Sox2 on the proliferation of bladder cancer cell line T24. Methods:Immunohistochemical and immunofluorescence staining was used to detect the expression of Sox2 in bladder cancer. The constructed pCDNA3.1-SOX2-EGFP plasmid was transfected into bladder cancer cell line T24. The expression of Sox2 in T24 was analyzed by RT-PCR and Western blot. The function of Sox2 protein in cell proliferation was measured by MTT assay. Results:The high expression of Sox2 was detected in 68.6% of bladder cancer specimens. The pCDNA3.1-SOX2-EGFP plasmid was successfully constructed. In addition, MTT assay showed that Sox2 could significantly promote the proliferation in human bladder cancer cell line T24. Conclusion:The expression of Sox2 was detected in bladder cancer. Sox2 might promote the growth of human bladder cancer cell line T24.