线粒体解偶联蛋白2在京尼平促进肾癌细胞抑制作用中的研究
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贵州省科技厅社会攻关计划项目(黔科合sy字[2011]3060);贵阳市科技计划项目(筑科合同[2011103]26号)


The effect of mitochondrial uncoupling protein 2 on suppression of genipin on renal cell carcinoma
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    摘要:

    目的:探讨京尼平(Genipin)对肾癌是否有治疗作用,该治疗作用是否与线粒体解偶联蛋白2(UCP2)相关。方法:将0-40-80和120 μmol/L 4种浓度的Genipin溶液分别浸染肾癌细胞48 h,MTT法和流式细胞术检测细胞的增殖和凋亡情况;聚合酶链反应和ELISA方法检测UCP2的基因和蛋白表达;使用荧光探针检测细胞内钙离子和活性氧分子(ROS)含量。结果:Genipin在中-高剂量组能明显抑制肾癌细胞的增殖并促进细胞的凋亡,随着给药浓度的增加,细胞增值抑制作用及细胞凋亡率逐渐增高(P < 0.005)。Genipin能抑制肾癌细胞中UCP2的表达,在中-高剂量给药组,UCP2的基因及蛋白的表达显著抑制(P < 0.05)。Genipin能提高细胞内钙离子和ROS的含量,在中-高剂量给药组出现了显著提高(P < 0.05)。结论:Genipin能明显抑制肾癌细胞的增殖,促进其凋亡,UCP2可能参与其作用机制。

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    Objective:To investigate the therapeutic effect of genipin on renal cell carcinoma,and the relationship of UCP2. Methods:Renal carcinoma cells were treated with different concentrations(0,40,80,120 μmol/L)of genipin. Cell growth inhibition and apoptosis were detected by assay of MTT and flow cytometry. Expression of UCP2 was detected by PCR and ELISA. Contents of ROS and Ca2+ were tested by fluorescence probe. Results:Genipin significantly suppressed the growth and promoted apoptosis rate of renal carcinoma cells,statistical significance were found in 80 and 120 μmol/L groups. Expression of UCP2 was significantly increased in 80 and 120 μmol/L groups. Genipin improved contents of ROS and Ca2+,and statistical significance were found in 80 and 120 μmol/L groups also. Conclusion:Genipin can suppress renal carcinoma cells growth,and UCP2 may be involved in its mechanism.

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杨永安,谷 江,张永春,王 楠,朱致晖,杨清涛,祝庆亮.线粒体解偶联蛋白2在京尼平促进肾癌细胞抑制作用中的研究[J].南京医科大学学报(自然科学版),2013,(4):444-448

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  • 收稿日期:2012-11-26
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  • 在线发布日期: 2013-04-29
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