Objective:To investigate the effects of wogonin on human glioblastoma cell lines U87,and the role of miR-128 in the mechanism of wogonin effect on U87 cells. Methods:U87 cells were treated by wogonin within three different concentrations(25,50,100 μmol/L) for 24 h,48 h,respectively. The morphological change of U87 cells were observed by using fluorescence microscope. The MTT was used on cell proliferation test. Flow cytometry(FCM) was performed to analyze the cell cycle and cell apoptosis. The qRT-PCR of miR-128 was detected from U87 cells after treated with or without wogonin. Results:Obvious appearances of cell apoptosis were observed under fluorescence microscope,including chromatin condensation and nuclear fragmentation. The analysis of MTT assay showed that the cell proliferation of U87 was inhibited by wogonin and associated with dose- and time-dependent manner. The FCM showed that the U87 cells treated by wogonin were arrest in S phase and the apoptosis ratio of U87 treated by wogonin was significantly increased after 24 h. The expression of miR-128 increased after being treated with wogonin. Conclusion:Wogonin induces glioblastoma cell U87 apoptosis and may restrain cell proliferation through miR-128.