IL-17在甲状腺乳头状癌中的表达与临床意义
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国际科技合作计划—国际产学研合作项目(BZ2009053)


The expression of IL-17 in papillary thyroid carcinoma and its clinical significance
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    摘要:

    目的:研究白细胞介素-17(IL-17)在人甲状腺乳头状癌组织中的表达,探讨IL-17在甲状腺乳头状癌发生发展中的作用。方法:采用real-time RT-PCR-Western blot-免疫组织化学法检测IL-17在60例甲状腺乳头状癌-10例结节性甲状腺肿和10例桥本氏病组织中的表达,应用统计学方法对其结果进行分析。结果:①甲状腺乳头状癌IL-17 mRNA水平高于结节性甲状腺肿,而低于桥本氏病,其中癌旁组织高于癌组织,P < 0.05;②Western blot检测结果显示,IL-17蛋白在结节性甲状腺肿基本不表达,甲状腺乳头状癌癌旁组织表达高于癌组织,但均低于桥本氏病(P < 0.05);③免疫组化染色显示IL-17在甲状腺乳头状癌和桥本氏病均有表达,结节性甲状腺肿无表达,且甲状腺癌染成棕色的区域主要位于肿瘤边缘;④临床资料统计显示IL-17的表达与甲状腺乳头状癌患者的年龄-性别-肿瘤个数无关,与肿瘤直径-淋巴结转移以及TNM分期相关。结论:IL-17在甲状腺乳头状癌表达增高,IL-17可能通过影响多种细胞因子以及炎症细胞参与甲状腺癌的发生和发展。

    Abstract:

    Objective:To investigate the expression of IL-17 in human thyroid papillary carcinoma tissues, and explore the role of IL-17 in the development of papillary thyroid cancer. Methods:Real-time RT-PCR,Western blot,immunohistochemistry assay were used to detect the expression of IL-17 in 60 cases of papillary thyroid carcinoma,10 cases of nodular goiter,and 10 cases of Hashimoto’s disease tissues. Statistics methods were applied to analyze the results. Results:①The level of IL-17 mRNA in papillary thyroid carcinoma tissues was higher than that in nodular goiter tissues,and lower than that in Hashimoto’s disease tissues. While While the level in paraneoplastic tissues was higher than that in the cancerous tissue,P < 0.05;②IL-17 protein in nodular goiter tissues was rarely seen,but expressed in papillary thyroid cancer,higher in adjacent tissues and highest in Hashimoto’s disease tissues by comparing the Western blot’s gray value;③Immunohistochemistry assay showed the expression of IL-17 in papillary thyroid cancer and Hashimoto’s disease tissues,and no expression in nodular goiter tissues. The dyed brown areas in thyroid cancer were mainly located on the edge of the tumor;④Clinical data statistics showed that the expression of IL-17 had no correlation with the patient’s age,sex,tumor numbers,but related with tumor size,lymph node metastasis and TNM staging. Conclusion:The expression of IL-17 increases in papillary thyroid carcinoma tissues. It may be involved in the occurrence and development of thyroid cancer by interacting a variety of cytokines and inflammatory cells.

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屠 燕,沈美萍,秦瑞浩. IL-17在甲状腺乳头状癌中的表达与临床意义[J].南京医科大学学报(自然科学版),2013,(4):485-489

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  • 收稿日期:2012-11-21
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  • 在线发布日期: 2013-04-29
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