Objective:To investigate the drug resistance of Klebsiella pneumonia in ventilator associated pneumonia in pediatric intensive care unit (PICU) in Suzhou,and provide the basis of prevention and clinical treatment. Methods:Children recruited in PICU from Aug. 2011 to Jan. 2012 who met the diagnostic criteria of ventilator associated pneumonia(VAP) and sputum culture showed Klebsiella pneumonia positive were studied. According to extended-spectrum β-lactamases(ESBLs),they were divided into two groups:ESBLs positive and ESBLs negative. The difference of drug resistance was analyzed. Results:Totally 39 sputum specimens from 31 children were collected and under susceptibility test. All strains showed ampicillin resistance,32 strains showed eftazidime resistance,the drug resistance rate was increased to 82.05% (32/39). Other antibiotics resistances were cefuroxime axetil (89.74%,35/39),cefepime (76.92%,30/39) and cefotaxim (76.92%,30/39). Cefoxitin had a lower resistance (17.95,7/39). All strains were sensitive to imipenem. The drug resistance(of the 2nd,3rd and 4th generation of cephalosporin) rate in the ESBLs positive group was higher than that in the ESBLs negative group(P < 0.05). Conclusion:ESBLs positive strains are more common in VAP Klebsiella pneumonia infection in PICU in Suzhou,and have a higher cephalosporin resistance compared to the ESBLS negative strains. Carbapenem is the first choice drug to treat VAP Klebsiella pneumonia infection. Doctors should choose suitable antibiotics according to the resistance.