Objective:For guiding the clinical therapy,we sought to analyze the changing of department distribution and the resistance of Acinetobacter baumannii from the First Affiliated Hospital of NJMU during 2008 to 2011 Methods:The bacteria were identified by VITEK2 identification system or API bacterial identification strip. Antimicrobial susceptibility testing were carried out by the disc diffusion method (Kirby-Bauer method). Data analyses were performed by Whonet 5.6 software. Results:Acinetobacter baumannii ranked the highest proportion (80.0%) in sputum samples. The bacteria was detected in some clinical departments,including geriatrics,ICU,emergency department,respiratory and neurosurgery. The resistance rates to cefoperazone/sulbactam,imipenem and meropenem among Acinetobacter baumannii in 2008 were 12.0%,14.8% and 23.3%,respectively;while the resistance rates in 2011 increased to 67.4%,90.8% and 91.1%,respectively. Conclusion:We found that the resistance rates of many clinical used antimicrobial agents significantly increased in Acinetobacter baumannii isolates. We should pay attention to the antimicrobial resistance monitoring and control its spread.