Abstract:Objective:To observe the effect of Celastrol on the expressions of RANKL,OPG,IL-6,TNF-α and IL-8 in human rheumatoid synoviocyte MH7A. Methods:MH7A was stimulated with IL-1β and added in different concentration of Celastrol(0.1,0.5,1,2 μmol/L). The expressions of RANKL,OPG,IL-6,TNF-α and IL-8 in MH7A were assayed by real-time PCR and immunofluorescence. Results:The expressions of RANKL,OPG,IL-6,TNF-α and IL-8 in MH7A were significantly increased in MH7A upon stimulation with IL-1β(P < 0.05). Celastrol could inhibit the expressions of RANKL,OPG,IL-6,TNF-α and IL-8 in a dose-dependent manner and notably elevated the ratio of OPG/RANK axis. Conclusion:Celastrol could regulate the ratio of OPG/RANKL axis and suppress the inflammatory cytokines and chemokine expression in rheumatoid arthritis synovioblast,suggesting a potent role in preventing the inflammation and bone erosion in rheumatoid arthritis.
