Objective:In order to establish different mouse models of experimental autoimmune encephalomyelitis (EAE),compare the features of development,clinical scores and pathological analysis in different gene background. Methods:Mice were induced by myelin oligodendrocyte glycoprotein (MOG35-55) peptide in complete Freund’s adjuvant (CFA),also received pertussis toxin twice for immune enhancement ,established EAE mice models with record the onset time ,clinical manifestation,nerves function scores for daily. Finally the spinal cord and brain tissues were taken for pathological analysis with CD4 and IL-7 immunocytochemistry staining. Results:C57BL/6 mice reached the peak of clinical scores at 17 to 25 days after first induction,and showed typical performance like limp tail and partial or complete limb paralysis. The mean clinical score was 3.0. CD-1 mice put off the peak till 35 to 40 days after first induction compared with C57BL/6 mice. CD-1 mice also showed limp tail or limb paralysis with similar performance. The mean clinical score was 2.8. The results of H.E. and Luxor fast blue analysis showed CD-1 mice changing were lighter than C57BL/6 mice,most manifestations occurred in spinal cord. While immunochemistry analysis results showed CD4+ and IL-17+ lymphocytes infiltration in varying degrees. Conclusion:Different gene background have apparently influence on susceptibility,clinical performance and pathological changing in EAE model. CD-1 mice can also be used to produce chronic EAE model,with the characteristics coincide with multiple sclerosis.