Objective:In order to guide clinical doctor to select antimicrobial agents rationally,we sought to analyze the pathogen distribution and resistance pattern of positive clinical specimens from the department of gerontology of our hospital in 2011. Methods: The bacteria and fungi were identified by API or Vitek 2 compact system. The susceptibility of antimicrobial and antifungal agents was tested by K-B and the data were analyzed by WHONET5.6 software. Results:A total of 993 strains were isolated and collected in the department of gerontology,including 657 strains (66.2%) of Gram-negative bacilli,124 strains (12.5%) of Gram-positive cocci and 119 fungi(12.0%). The pathogen of the highest isolating rate was Pseudomonas aeruginosa,which accounted for 25.6%,the next was Acinetobacter species(19.0%) as well as Klebsiella(12.7%). Resistance analysis showed that among nonfermenters,Pseudomonas aeruginosa was resistant to the most of the antimicrobial agents,and Acinetobacter for most antimicrobial resistant was more than 70.0%,however for the resistance pattern of imipenem and meropenem were 88.7% and 91.1% respectively. Meanwhile,Escherichia coli were resistant to the most of cephalosporins,but for the resistance pattern of imipenem and meropenem were 0.0% and 1.5%,respectively. The resistance pattern of Klebsiella was higher than Escherichia coli,and for the resistance pattern of imipenem and meropenem were 35.2% and 42.3%,respectively. As for Staphylococcus species,none was found to resistant to vancomycin. However,they were resistant to many other antimicrobial agents. Conclusion:The pathogens in our department of gerontology have generated diversity,and the resistance pattern of the pathogens is rising. Especially,the resistance to carbapenems among Acinetobacter species and Enterobacteriaceae were serious,and we need to pay more attention on this issue.