Calcineurin和TRPC6参与ET-1诱导的肺动脉平滑肌细胞增殖
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国家自然科学基金资助(81070045);卫生部部属(管)医院临床学科重点项目(111)


Calcineurin and TRPC6 are involved in ET-1-induced proliferation of pulmonary artery smooth muscle cells
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    摘要:

    目的:探讨Calcineurin和瞬时感受器电位6(TRPC6)的相互作用以及对内皮素-1(ET-1)诱导的肺动脉平滑肌细胞增殖的影响。方法:以 ET-1刺激肺动脉平滑肌细胞(PASMCs)检测Calcineurin活性以及TRPC6的表达;分别于ET-1刺激前给予Calcineurin特异性抑制剂环孢素A(CsA)和TRPC6 siRNA处理,测定Calcineurin活性-TRPC6的表达以及细胞的增殖情况;采用Calcineurin活性检测试剂盒检测其活性,Western blot和RT-PCR检测TRPC6的表达,MTT法检测PASMCs的增殖。结果:ET-1可激活原代培养的PASMCs中Calcineurin的活性,上调TRPC6的表达;CsA和TRPC6 siRNA可以分别降低TRPC6表达和Calcineurin活性;也可以抑制ET-1 刺激的 PASMCs增殖。结论:Calcineurin与TRPC6参与ET-1诱导的肺动脉平滑肌细胞增殖并相互影响。

    Abstract:

    Objective:To explore the interaction between Calcineurin and TRPC6, and the impact of this interaction on endothelin-1(ET-1)-induced proliferation of pulmonary artery smooth muscle cells (PASMCs). Methods:PASMCs were treated with ET-1 to stimulate their proliferation with or without prior treatment of CsA or TRPC6 siRNA. Calcineurin activity in the cells was measured using a calcineurin activity assay kit,and TRPC6 expression was examined using Western blotting and RT-PCR. PASMCs proliferation following the treatments was determined using MTT assay. Results:ET-1 activited Calcineurin activity and upregulated TRPC6 expression. TRPC6 expression was decreased in response to the CsA treatment. Calcineurin activity was attenuated after pretreatment with TRPC6 siRNA;however,ET-1-stimulated PASMCs proliferation was inhibited after pretreatment with both CsA and TRPC6 siRNA. Conclusion:Calcineurin and TRPC6 are involved in ET-1-induced proliferation in PASMCs,and the interaction existes between Calcineurin and TRPC6 in PASMCs.

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王小闯,李满祥,党晓燕,李 萍,彭 卓,高彦霞,古长维. Calcineurin和TRPC6参与ET-1诱导的肺动脉平滑肌细胞增殖[J].南京医科大学学报(自然科学版),2013,(9):1191-1195

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  • 收稿日期:2013-04-24
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  • 在线发布日期: 2013-09-18
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