Objective:To establish a culture method for primary mouse subcutaneous and visceral adipocyte precursor cells. Methods:Fibroblast-like cells were collected from C57BL/6J mice inguinal and epididymal adipose tissue. The morphological changes of the cultured cells were observed,the intracytoplasmic lipid of the culture cells was determined using oil red O staining,and the expression of the specific adipogenic gene was determined by real time PCR. Results:The cultured fibroblast-like cells showed highly homogeneous appearance with active proliferation and differentiate into mature adipocytes. Oil red O staining,morphological observation,and expression of specific adipogenic gene Fabp4 verified these cells as adipocyte. Conclusion:Adipocyte precursor cells are presented in C57BL/6J mice inguinal and epididymal adipose tissue and possess the potential to proliferate and differentiate into mature adipocyte. The establishment of primary culture of mouse subcutaneous and visceral adipocyte precursor cells is important for further studies of the differences between subcutaneous and visceral adipocyte in vitro.