Objective:To study the genetic polymorphism,frequencies and genetic distance of 24 Y-chromosomal short tandom repeat(STR) loci in unrelated individuals of Han nationality in Jiangsu province. Methods:Twenty-four STR loci were amplified in DNA samples from 262 unrelated individuals in Jiangsu Han population from 2011 to 2012. All samples of the PCR products were genotyped by ABI 3130 Genetic Analyzer. Results:In total,172 genotypes and 262 haplotypes were founded,DYS385b,DYS449 loci had 14 genotypes,the diversity of overall heliotypes for the 24 Y-STR loci was 0.999 9. Comparing with 9 other populations,the genetic distance was more closed between Jiangsu and Tianjin (-0.001 8),Henan (-0.001 76),Shanxi (-0.001 6) province,compared to Hunan(0.017 54) and Liaoning(0.014 64) population(P < 0.05). Conclusion:We obtained objective basic data in the area of population genetics and forensic medicine,which would be useful for building Y-STR database in Jiangsu Han population.