Objective:To explore the risk factors of patients with type 2 diabetes mellitus (T2DM ) with non-alcoholic cirrhosis (NAFLD). Methods:The enzyme-linked immunosorbent assay (ELISA) and chemiluminescene analyzer were used to detect the serum cytokeratin 18(CK18),free fatty acids (FFA),tumor necrosis factor alpha (TNF-α),superoxide dismutase enzyme (SOD) in 45 cases of T2DM,44 cases of T2DM+NAFLD patients and 24 cases of control. Body mass index(BMI),insulin resistance index(HOMA-IR),fasting plasma glucose (FPG) and general physiological and biochemical indexes of the study group and the control group were simultaneously detected and calculated,and their correlation with NAFLD was analyzed by correlation analysis and binary logistic regression statistical analysis. Results:BMI,HOMA-IR,FPG,TC,TG,AST,ALT,GGT,FFA,CK18 and TNF-α were positively related with NAFLD. SOD was negatively related with NAFLD. FINS and HbA1c have no relation with NAFLD. BMI,HOMA-IR,FFA and CK18 were highly related with etiology of NAFLD. Conclusion:The pathogenesis of NAFLD is the result of multiple factors. The BMI,HOMA-IR,FFA and CK18 is independent risk factor of the NAFLD.