Objective:To analysis the value of single-photon emission tomography/computed tomography(SPECT/CT ) in diagnosing parathyroid in patients with hyperparathyroidism. Methods:Thirty-five patients with secondary hyperparathyroidism (SHPT) were performed 99mTc-methoxyisobutylisonitrile(99mTc-MIBI) dual-phase planar scintigraphy (MIBI planar),and 46 focis outside of thyroid-bed were observed. SPECT/CT imaging including neck and additional radical uptake was added in them. The difference in sensitivity and false positive percent(FPP) between MIBI planar and SPECT/CT in finding the normalsite parathyroid and ectopic parathyroid were compared according to the pathology. Results:The sensitivity of MIBI planar in finding the normalsite parathyroid was similar to SPECT/CT(63.9% vs 67.0%,P > 0.05),the FPP was significantly lower than SPECT/CT(3.0% vs 18.4%,P <0.05). The sensitivity of MIBI planar in finding the ectopic parathyroid was similar to SPECT/CT(either 92.5%),the FPP was significantly lower than SPECT/CT(2.6% vs 19.6%,P < 0.05). The sensitivity of SPECT/CT in finding the ectopic parathyroid was significantly higher than itself in finding the normalsite parathyroid(92.5% vs 67.0%,P < 0.05). Conclusion:SPECT/CT can not add the sensitivity in finding parathyroid,but can significantly reduce FPP. It is very valuable in clinical work,especially in finding the ectopic parathyroid.