Objective:To investigate the relationship between serum 25-Hydroxy Vitamin D and lipid profiles among middle-aged and elderly population. Methods:Individuals of 724 were enrolled in this study. Fasting blood samples were collected and serum 25-Hydroxy Vitamin D and biochemical indices were determined. Subjects were divided into three groups (T1-T2-T3) according to tertiles of 25-Hydroxy Vitamin D levels. ANOVA was used to detect the difference among the three groups. Multiple linear regression was conducted to investigate relationships between 25- Hydroxy Vitamin D levels and lipid profiles. Results:There was significant difference among three groups for TG,but not for TC,HDL and LDL. 25-Hydroxy Vitamin D was associated with TG in total population(regression coefficient -0.013,P < 0.001). After adjustment for age,BMI,fasting blood glucose,HbA1c,glutamic transaminase,and γ-glutamyl transpeptidase,the association was still significant in all subjects and females. Conclusion:Middle-aged and elderly individuals with lower serum 25-Hydroxy Vitamin D had higher TG level. 25-Hydroxy Vitamin D is associated with TG negatively,and particularly in females. However there was no significant association between 25-Hydroxy Vitamin D and TC,HDL or LDL.