烟碱对膝骨关节炎模型大鼠的保护作用及机制研究
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国家自然科学基金(30901800)


Chondroprotective effects and mechanisms of nicotine on osteoarthritis in rat models
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    摘要:

    目的:研究烟碱对碘乙酸(monoiodoacetic acid,MIA)诱导的大鼠膝骨关节炎模型的保护作用,并对其作用机制作初步探讨-方法:通过MIA膝关节腔注射法构建大鼠膝骨关节炎模型,将造模成功的30只SD大鼠随机分为模型组(MIA组)10只,烟碱干预组20只(MIA+Nic 0.25 mg/kg与MIA+Nic 0.5 mg/kg组各10只)-另外,将10只大鼠作为假手术组(对照组)-造模30 d后提取大鼠右膝关节软骨组织标本,进行大体光镜和组织学病理切片观察关节软骨破坏情况;并用RT-PCR法对各组大鼠右膝关节软骨内的Ⅱ型胶原-蛋白聚糖的表达进行检测;采用Western blot法检测磷酸化Akt蛋白表达-结果:与MIA组相比,烟碱可明显改善模型大鼠膝关节大体光镜评分和Mankin’s评分(P < 0.01),提高关节软骨Ⅱ型胶原和蛋白聚糖表达水平(P < 0.01),并显著增加磷酸化Akt蛋白水平(P < 0.01)-结论:烟碱可有效缓解MIA诱导的大鼠膝关节软骨退变,这种保护作用可能与PI3K/Akt信号途径有关-

    Abstract:

    Objective:To study the protective effects of nicotine on cartilage of monoiodoacetic acid(MIA)-induced rat osteoarthritis model. Methods:A total of 40 rats were randomly divided into four groups(control group,MIA group,MIA+Nic 0.25 mg/kg group and MIA+Nic 0.5 mg/kg group). Each group rats were sacrificed on 30th day after they received the first intra-articular injection of MIA(1mg) surgery. The right knee samples were carefully dissected. Generally light microscope and histological pathological examination were used to observe the destruction of articular cartilage. Reverse-transcription PCR was used to detect the gene expression of type II collagen and aggrecan. The protein expression of phosphor-Akt was measured by Western blot test. Results:Compared with the MIA group,nicotine treatment significantly decreased the light microscopy score and the Mankin’s score(P < 0.01) of the knee samples,and significantly increased the mRNA levels of typeⅡ collagen and aggrecan (P < 0.01) and the protein levels of phosphor-Akt (P < 0.01). Conclusion:Nicotine attenuates the severity of cartilage degeneration in the experimental rat model. These protective effects may partially benefit from the increase of phosphor-Akt expression.

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朱辰蕾,惠宇坚,张翔海,吴东迎,范卫民,胡 军.烟碱对膝骨关节炎模型大鼠的保护作用及机制研究[J].南京医科大学学报(自然科学版),2014,(2):129-134

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  • 收稿日期:2013-05-13
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  • 在线发布日期: 2014-03-03
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