miR-638对人肺腺癌细胞凋亡的影响
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国家自然科学基金(81371894,81302531, 81272324,81201359,81101322);江苏省实验诊断学重点实验室(XK201114);江苏高校优势学科建设工程基金项目;教育部博导基金(20113234110012);国家临床重点专科建设项目


Impact of miR-638 on apoptosis of lung adenocarcinoma cells
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    目的:探讨miR-638在肺腺癌中的表达及其与肺腺癌细胞凋亡的关系-方法:应用real-time RT-PCR的方法检测了miR-638在肺腺癌细胞株SPC-A1中的表达情况,并采用脂质体法将miR-638 模拟物瞬时转染入SPC-A1-实验设置空白对照组-无关miRNA阴性对照组和miR-638转染组,转染后在荧光显微镜下观察转染效率,实时荧光定量RT-PCR检测miR-638的表达,流式细胞术检测各组细胞凋亡率-结果:与正常细胞相比,miR-638在肺腺癌细胞中低表达-SPC-A1细胞转染miR-638模拟物后,细胞凋亡率相对于空白组和阴性对照组显著升高(P < 0.05)-结论:miR-638在肺腺癌中低表达,且能够促进肺腺癌细胞凋亡,可作为后续肺癌生物治疗的新分子靶标-

    Abstract:

    Objective:To investigate expression of miR-638 and effects of miR-638 on apoptosis of lung adenocarcinoma cell line(SPC-A1). Methods:The expression of miR-638 in lung adenocarcinoma cell line was detected by real-time RT-PCR. Mimics of mir-638 were transiently transfected into SPC-A1 by using lipofectamine method. SPC-A1 cells were transfected with miR-638 mimics or non-special oligonucleotides(as negative control)or nothing(as blank control). After transfection,transfection efficiency was observed by fluorescence microscope. MiR-638 levels were detected by real-time quantitative RT-PCR. Cell apoptosis was detected by flow cytometry. Results:We observed that miR-638 was significantly inhibited in lung adenocarcinoma cells compared with that in normal cells. Cell apoptosis rate was increased significantly in cells transfected with miR-638(P <0.05)compared with negative and blank control cells. Conclusion:MiR-638 was poorly expressed in lung adenocarcinoma cells,and it could dramatically promote apoptosis of lung adenocarcinoma cells,thus provides a new target for the use of miR-638 in lung cancer biotherapy.

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曹 艳,王 鹏,娄鉴芳,李大千,吴 蕾,陈 丹,谢而付,顾 兵,徐华国,王 芳,徐 建,潘世扬. miR-638对人肺腺癌细胞凋亡的影响[J].南京医科大学学报(自然科学版),2014,(3):287-290

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  • 收稿日期:2013-12-19
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  • 在线发布日期: 2014-03-26
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