Objective:The aim of this study was to evaluate the potential relationship between single nucleotide polymorphisms/haplotypes of beta-2 adrenergic receptor (ADRB2) gene and asthma in a Han Chinese population. Methods:Six loci (-2 387,-47,46,79,491 and 523 loci) of ADRB2 gene were genotyped by the TaqMan probe assay in 379 asthmatics and 435 healthy controls,and the haplotypes were also analyzed by the SHEsis online platform. Results:There was significant difference in allele frequency distribution of -47 locus between asthmatic patients and control subjects (OR:0.687,95%CI:0.497~0.951,P < 0.05). Compared with TT genotype,the CT and CC + CT genotype both had significant difference (OR were 0.685,0.672,respectively;95%CI were 0.480~0.976,0.474~0.952,respectively;both P < 0.05). There was also significant difference in allele frequency distribution of 79 loci between asthmatic patients and control subjects (OR:0.687,95%CI:0.497~0.951,P < 0.05). Compared with the CC genotype,the CG and CC + CG genotype both had significant difference (OR were 0.672,0.687,respectively;95%CI:0.480~0.976,0.474~0.952,respectively;both P < 0.05). There were no significant differences in genotype or allele frequencies of loci of -2 387,46 and 523 between the two groups (all P > 0.05). The mutant allele at 491 locus was not observed and only the homozygous wild-type was found in both asthmatic patients and control subjects. A strong linkage disequilibrium was found between -47 and 79 loci (D＇ = 0.987,r2 = 0.974) and between 46 and 523 loci (D＇ = 0.996,r2 = 0.593). There was significant difference in the haplotype Ⅲ (CCGGC) distribution frequency between asthmatic patients and control subjects (OR = 0.696,95%CI:0.502~0.966,P < 0.05). Conclusion:The polymorphisms of -47,79 loci and the haplotypeⅢ(CCGGC) of ADRB2 gene may contribute to susceptibility of asthma in Chinese Han population. There were no association between the polymorphisms/haplotypes of -2387,46,491,523 loci and asthma susceptibility.