Objective:To explore VEGF expression induced by PGE2 in endometrial cancer cell and possibly involved signal transduction pathways. Methods:Ishikawa cells were treated with PGE2,EP1-4 receptor agonist,EP2 receptor antagonist AH6809,PKA inhibitor H89,and adenylate cyclase (AC) inhibitor SQ22536. Western blot was used to examine the expression of VEGF protein in Ishikawa cells cells. Results:PGE2 up-regulated the protein level of VEGF in Ishikawa cells. The level of VEGF protein was increased by 31.56%(P < 0.05)compared with the control group after treated with 10 μM PGE2 for 24 h. Treated with 10 μM four selective EP receptor agonists(17-phenyltrinor Prostaglandin E2,Butaprost,Sulprostone and Prostaglandin E1 Alcohol) respectivly,the protein expressions of VEGF in Ishikawa cells was increased compared with the control group. We found that EP2 receptor agonist increased the expression of VEGF protein to 87.8% (P < 0.05). The protein level of VEGF decreased by 45.66%(P < 0.05)after treated with 10 -滋M EP2 receptor antagonist compared with the group treated with PGE2. When treated with 25 -滋M AC inhibitor SQ22536 and 10 -滋M PKA antagonist H89 for 24 h,respectally,the protein levels of VEGF were decreased by 29%(P < 0.05)and 57.5%(P < 0.05)compared with the group treated with EP2 receptor agonist. Conclusion:PGE2 may up-regulate the protein expression of VEGF through EP2 receptor and Gαs-cAMP-PKA signaling pathway in Ishikawa cells.