鱼藤素对小细胞肺癌的体外抗肿瘤效果评价及相关机制分析
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国家自然科学基金(81273571)


The antitumor activities and mechanisms of deguelin in small cell lung cancer in vitro
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    目的:研究鱼藤素对小细胞肺癌体外抗肿瘤效果-方法:0-5-10-20-50 μmol/L鱼藤素作用于NCI-H446细胞24 h,细胞计数试剂盒8(cell counting kit-8,CCK8)分析鱼藤素对细胞活力的影响-据CCK8结果,以0-5-10-20 μmol/L鱼藤素作用于NCI-H446细胞,EdU掺入法检测细胞增殖,DAPI染色及流式细胞术测定细胞凋亡-据流式结果,20 μmol/L鱼藤素作用NCI-H446细胞0-0.5-2.0-4.0 h,蛋白质印迹法检测细胞内凋亡相关蛋白的表达-结果:鱼藤素呈剂量依赖性地抑制小细胞肺癌NCI-H446细胞增殖-诱导其凋亡,作用不同时间后可上调促凋亡蛋白Bax-下调抗凋亡蛋白Bcl-2水平-结论:鱼藤素体外抗小细胞肺癌效应可能通过上调促凋亡蛋白Bax-下调抗凋亡蛋白Bcl-2表达,诱导细胞凋亡实现-

    Abstract:

    Objective:To investigate the antitumor activity of deguelin in small cell lung cancer and the possible underlying mechanisms. Methods:NCI-H446 cells were treated with deguelin at 0,5,10,20 and 50 μmol/L for 24 hours. The effect of deguelin on cell activity of NCI-H446 cells was evaluated by CCK8. EdU assay and DAPI staining were performed to assess cellular proliferation and apoptosis,respectively. According to flow cytometry analysis,20 μmol/L deguelin were given NCI-H446 cells for 0,0.5,2.0 and 4.0 hours,respectively. Western blot was applied to detect the levels of proteins involved in apoptosis. Results:Deguelin significantly inhibited proliferation and induced apoptosis of NCI-H446 in a dose-dependent manner. After treating with deguelin for different time lengths,pro-apoptotic protein Bax level was increased and anti-apoptotic protein Bcl-2 level was decreased. Conclusion:Deguelin suppresses small cell lung cancer in vitro via promoting its apoptosis probably by up-regulating pro-apoptotic protein Bax and down-regulating anti-apoptotic protein Bcl-2.

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谢小晨,丁文秋,徐华娥,李晓林,曾晓宁,孔 辉,解卫平.鱼藤素对小细胞肺癌的体外抗肿瘤效果评价及相关机制分析[J].南京医科大学学报(自然科学版),2014,(6):721-726

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  • 收稿日期:2014-01-10
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  • 在线发布日期: 2014-06-19
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