Objective:To prepare long-circulating lipid membrane ultrasound contrast agent suitable for the ultrasonic diagnosis of inflammatory bowel disease (IBD) and targeted mucosal addressin cell adhesion molecule-1 (MAdCAM-1),and determine the physical characteristics of the microbubbles and investigate their affinity for SVEC4-10 cells in vitro. Methods:The long-circulating lipid membrane microbubbles were prepared by sonic dispersion,and bridged the microbubbles and anti-murine MAdCAM-1 monoclonal antibody (MECA-367) by “biotin-avidin”. The physical traits of prepared targeted microbubble contrast agent were detected. Its ability to target to IBD cell model induced by tumor necrosis factor-α (TNF-α) was determined under light microscope and confocal laser scanning microscope,and isotype control antibody IgG2a microbubbles were used as control. Results:The targeted ultrasound microbubbles had a good shape and uniform particle size,with the average diameter of (464.5 ± 85.7)nm,and the surface Zeta potential was (-19.3 ± 2.5)mV. MAdCAM-1 expression on cultured SVEC4-10 cells was positively correlated with the time of TNF stimulation. In addition,when the concentration of TNF-a was 20 ng/ml,MAdCAM-1 expression reached its peak. The targeting study in vitro showed that many targeted ultrasound contrast agents adhered more firmly to the surrounding cells expressing MAdCAM-1,while the control group had no significant binding. Conclusion:Bridging MECA-367 long-circulating lipid membrane ultrasound contrast agents were successfully prepared. The targeted ultrasound contrast agents can bind to the cell model of high expression MAdCAM-1 effectively in vitro,and the method of establishing IBD cell model on TNF-stimulated SVEC4-10 cells is simple and feasible.