HGF经HGF/Akt通路抑制肝癌细胞HCCC-9810的增殖
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江苏省苏北人民医院院级科研基金项目(yzucms201318)


HGF inhibited the proliferation of liver carcinoma cell line HCCC-9810 via HGF/Akt signaling pathway
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    目的:观察肝细胞生长因子(HGF)对人胆管细胞型肝癌细胞HCCC-9810增殖的影响,并探讨其分子机制-方法:HGF处理HCCC-9810细胞后,用MTT及EdU法检测癌细胞的增殖;siRNA干扰技术抑制HCCC-9810细胞内源性Akt的表达,Western blot检测癌细胞Cyclin D1蛋白表达和Akt磷酸化的变化-结果:50和100 ng/ml HGF显著抑制肝癌细胞HCCC-9810的增殖-Western blot结果显示,HGF处理后的肝癌细胞Akt磷酸化和Cyclin D1表达量明显下调-siRNA干扰Akt的表达,阻断了HGF诱导的Cyclin D1表达量的下调及细胞增殖抑制-结论:HGF通过下调Akt磷酸化和Cyclin D1表达量抑制肝癌细胞HCCC-9810的增殖-

    Abstract:

    Objective:To identify the novel function of hepatocyte growth factor (HGF) in liver carcinoma cell HCCC-9810 proliferation and the underlying molecular mechanism. Methods:The proliferation of HCCC-9810 treated with HGF was detected by MTT assay and EdU assay. The expression of Akt in HCCC-9810 cells was inhibited by siRNA. Western blot was used to detect the expression of cyclin D1 and the phosphorylation level of Akt. Results:Both 50 ng/ml and 100 ng/ml HGF significantly inhibited the proliferation of HCCC-9810. Western blotting assay showed that HGF downregulated the expression of cyclin D1 and the phosphorylation level of Akt in HCCC-9810 cells. Conclusion:These results suggest that HGF inhibited the proliferation of HCCC-9810 by suppressing the expression of cyclin D1 and the phosphorylation level of Akt. SiRNA interfered the expression of Akt,and blocked HGF-induced downregulation of cyclin D1 expression and inhibition of cell proliferation.

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王广洲,周 林,吴银霞. HGF经HGF/Akt通路抑制肝癌细胞HCCC-9810的增殖[J].南京医科大学学报(自然科学版),2014,(7):870-874

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  • 收稿日期:2013-12-10
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  • 在线发布日期: 2014-07-05
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