YY1在胃癌组织中的表达及其对SGC-7901细胞株增殖和凋亡的影响
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国家自然科学基金(81361120398,81072031, 81272712)


Expression of YY1 in gastric cancer and its effect on the proliferation and apoptosis of gastric cancer cell line SGC-7901
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    摘要:

    目的:检测YY1在胃癌组织中的表达情况,探讨YY1对人胃癌细胞株SGC-7901增殖-凋亡的影响以及可能的机制-方法:利用定量PCR和免疫组织化学分别检测标本中YY1的mRNA和蛋白表达水平-利用慢病毒表达载体系统在胃癌细胞株SGC-7901中上调YY1,运用CCK-8-流式细胞术-平板克隆实验检测YY1对细胞的增殖-凋亡以及克隆形成能力的影响-结果:胃癌组织中YY1的mRNA和蛋白表达水平明显低于癌旁组织-与空载对照组(SGC-7901-Empty Vector,SGC-7901-EV)和与正常对照组(SGC-7901)相比,过表达YY1组细胞(SGC-7901-YY1)的增殖和平板克隆形成能力明显减弱,细胞凋亡增加-结论:YY1与胃癌的发生发展有一定的关系,可能起到抑癌基因的作用,YY1基因可能成为胃癌靶向治疗的新的潜在靶点-

    Abstract:

    Objective:To investigate the expression level of YY1 in gastric cancer,and then detect the effect of YY1 on the proliferation and apoptosis in SGC-7901 cell lines and its possible mechanism. Methods:mRNA and protein expressions of YY1 in cancer and paired adjacent tissues were detected by real-time PCR and immunohistochemistry,respectively. YY1 was up-regulated by a lentiviral vector expression system in gastric cancer cell line SGC-790. CCK-8 was performed to detect cell proliferation,and colony formation assay was performed to evaluate cell growth. Apoptosis was detected by flow cytometry. Results:The expression of YY1 in paired adjacent tissues had a higher level than that in tumor tissues. Compared to the empty vector group (SGC-7901-Empty Vector/SGC-7901-EV) and the normal control group (SGC-7901),the cell proliferation and clonogenicity were significantly decreased as well as cell apoptosis was increased in the over-expressed YY1 group (SGC-7901-YY1). Conclusion:YY1 may participate in the development of gastric cancer. YY1 can play a role as a cancer suppressor gene in the development of gastric cancer,which suggests that YY1 may be a new therapeutic target for gastric cancer.

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谢坤领,张静静,朱 毅,徐泽宽. YY1在胃癌组织中的表达及其对SGC-7901细胞株增殖和凋亡的影响[J].南京医科大学学报(自然科学版),2014,(8):1010-10,141,024

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  • 收稿日期:2014-03-09
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  • 在线发布日期: 2014-08-03
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