肢体远程预适应对大鼠急性肾损伤的保护作用及机制研究
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2006年烟台市科技发展计划(第一批)(2006131-1)


Protective effect and mechanism of limb ischemic preconditioning on acute renal injury in rats
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    摘要:

    目的:通过建立后肢缺血预适应大鼠模型,研究远程预适应对大鼠肾脏缺血-再灌注损伤保护作用的机制-方法:48只健康雄性SD大鼠摘除右肾后随机分为4组:假手术组(sham组)-单纯后肢预适应组(LIP组)-单纯肾缺血再灌注组(IR组)-后肢预适应 + 肾缺血组(LIP-IR组),再灌注24 h后检测血肌酐-尿素氮水平,肾组织病理学改变,肾组织中一氧化氮(NO)及一氧化氮合酶(NOS)活性,包括总NOS(TNOS)-诱导型NOS(iNOS)和结构型NOS(cNOS)-结果:与IR组相比,LIP-IR组血肌酐-尿素氮-肾小管评分,肾组织中TNOS-iNOS活性均明显降低,但肾组织NO-cNOS活性明显增高(P < 0.05);LIP组与sham组相比,上述指标无显著性差异(P > 0.05)-结论:肢体缺血预适应能够减轻大鼠肾脏缺血-再灌注损伤,其可能与LIP上调eNOS表达,同时降低肾脏iNOS而发挥肾保护作用有关-

    Abstract:

    Objective:To establish an animal model of hind limb ischemic preconditioning (RIPC) and to study the protective effect of remote ischemic preconditioning on renal ischemic reperfusion injury in rats. Methods:A total of 48 healthy Sprague Dawley rats were subjected to right nephrectomy,and then randomly divided into four groups:the sham operation (Sham),the simple hind limb ischemic preconditioning group (LIP),the simple renal ischemia reperfusion group (IR),and the hind limb ischemic preconditioning-renal ischemia reperfusion group (LIP-IR). After 24 h of reperfusion,serum creatinine (Scr) and blood urea nitrogen (BUN) were tested,pathological changes of renal tissues were observed,renal tissues were collected for the determination of NO and TNOS,iNOS and cNOS activity. Results:The level of Scr and BUN and the renal tubule injury score,and the activity of TNOS and iNOS in the kidney tissues were significantly decreased in the LIP-IR group as compared with those in the IR group (P < 0.05),but the level of NO and the activity of cNOS in the kidney tissues were significantly increased (P < 0.05). Compared with the sham group,the above indicators in the LIP group have no significant difference (P > 0.05). Conclusion:Limb ischemic preconditioning can protect kidney from ischemic reperfusion injury. The protection may be related to the increased expression of eNOS as well as the reduced expression of iNOS in the kidney.

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朱世慧,李 鹏,范 丽.肢体远程预适应对大鼠急性肾损伤的保护作用及机制研究[J].南京医科大学学报(自然科学版),2014,(8):1056-1059

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  • 收稿日期:2013-12-08
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  • 在线发布日期: 2014-08-03
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